Precise RNA targeting with CRISPR-Cas13d.
Hart SK, Muller S, Wessels HH, Mendez-Mancilla A, Drabavicius G, Choi O, Sanjana NE
Nat Biotechnol. 2025 Feb 11. doi: 10.1038/s41587-025-02558-3.
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PubMed
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228555 | pLentiRNACRISPR_010-TetO-NLS-RfxCas13d-2A-mCherry-NLS-WPRE-EFS-rtTA3-2A-Blast | Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus |
| 228557 | pLentiRNACRISPR_011-TetO-NLS-inactive-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast | Expresses inactive RfxCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus |
| 228558 | pLentiRNACRISPR_012-TetO-NLS-inactive-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast | Expresses inactive hfCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus |