Genetically encoded assembly recorder temporally resolves cellular histories in cellulo and in vivo
Yan Y, Lu J, Li Z, Zhao Z, Shay TF, Wang S, Lei Y, Wang Y, Chen W, Parker P, Yang H, Qi A, Sun Y, Bergles DE, Baker D, Lin D
bioRxiv 2025.07.16.664392
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228881 | YY120: pPB_TRE::GEMINI(B)-p2A-GEMINI(A) | PiggyBac plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a doxycycline-inducible promotor. |
| 228882 | YY105: pPB_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR | PiggyBac plasmid expressing the HaloTag-GEMINI(A) under the control of a doxycycline-inducible promotor, and rtTA3 and PuroR under the control of a UbC promoter. |
| 228883 | YY171: pPB_NFκB::GFP-GEMINI(A)_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR | PiggyBac plasmid expressing the GFP-GEMINI(A) under the control of a synthetic NFκB-responsive promotor, and rtTA3 and PuroR under the control of a UbC promoter. |
| 228884 | JL054: pPB_NFκB::GFP-GEMINI(A)-P1N4_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR | PiggyBac plasmid expressing the destabilized GFP-GEMINI(A) under the control of a synthetic NFκB-responsive promotor, and rtTA3 and PuroR under the control of a UbC promoter. |
| 228885 | YY203: pAAV_cfos::mCherry-GEMINI(A)-P1N4 | AAV plasmid expressing the destabilized mCherry-GEMINI(A) under the control of a cfos promotor. |
| 228886 | YY206: pAAV_hSyn::GEMINI(B)-p2A-GEMINI(A)_UbC::HaloTag-GEMINI(A) | AAV plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a hSyn promoter, and HaloTag-GEMINI(A) under the control of a UbC promoter. |
| 228888 | YY225: pAAV_TRE::GFP- GEMINI(A)-P1N4 | AAV plasmid expressing the destabilized GFP-GEMINI(A) under the control of a doxycycline-inducible promotor. |
| 228889 | YY229: pAAV_TRE::GFP- GEMINI(A)-P1N4_hSyn::rtTA3G | AAV plasmid expressing the destabilized GFP-GEMINI(A) under the control of a doxycycline-inducible promotor, and rtTA3G under the control of a hSyn promotor |