Marcin Suskiewicz Lab: Production and purification of SUMO-UBC9 and SUMO-RANGAP1CTD
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228947 | pET28a-RANGAP1-CTD | express the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimized |
| 228948 | pET28a-UBC9-K14R-A129K | express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K) |
| 228949 | pET28a-UBC9 | express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag) |
| 228951 | pET28a-shortSUMO1 | express a fragment of the human SUMO1 protein (residues 18-97 of UniProt P6316 with an N-terminal thrombin-cleavable tag, MGSSHHHHHHSSGLVPR|GSHM-, where | indicates the cleavage site). Codon-optimized |