Benchmarking and optimizing Perturb-seq in differentiating human pluripotent stem cells.
Sivakumar S, Wang Y, Goetsch SC, Pandit V, Wang L, Zhao H, Sundarrajan A, Armendariz D, Takeuchi C, Nzima M, Chen WC, Dederich AE, El Hayek L, Gao T, Ghazawi R, Gogate A, Kaur K, Kim HB, McCoy MK, Niederstrasser H, Oura S, Pinzon-Arteaga CA, Sanghvi M, Schmitz DA, Yu L, Zhang Y, Zhou Q, Kraus WL, Xu L, Wu J, Posner BA, Chahrour MH, Hon GC, Munshi NV
bioRxiv [Preprint]. 2025 Jan 23:2025.01.21.633969. doi: 10.1101/2025.01.21.633969.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229014 | Lenti-U6-sgRNA-BFP-Puro | Perturb Seq lentiviral sgRNA vector |
| 234076 | Donor-attp-u6-blank-sgrna-backbone | Blank donor for Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci |