Benchmarking and optimizing Perturb-seq in differentiating human pluripotent stem cells.
Sivakumar S, Wang Y, Goetsch SC, Pandit V, Wang L, Zhao H, Sundarrajan A, Armendariz D, Takeuchi C, Deng M, Nzima M, Chen WC, Dederich AE, El Hayek L, Gao T, Gogate A, Kaur K, Kim HB, McCoy MK, Niederstrasser H, Oura S, Pinzon-Arteaga CA, Sanghvi M, Schmitz DA, Yu L, Zhang Y, Zhou Q, Kraus WL, Xu L, Wu J, Posner BA, Chahrour MH, Hon GC, Munshi NV
Stem Cell Reports. 2025 Dec 9;20(12):102713. doi: 10.1016/j.stemcr.2025.102713. Epub 2025 Nov 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229013 | PB-U6-sgRNA-BFP-Puro | Perturb Seq sgRNA vector with PiggyBac backbone |
| 229014 | Lenti-U6-sgRNA-BFP-Puro | Perturb Seq lentiviral sgRNA vector |
| 234076 | Donor-attp-u6-blank-sgrna-backbone | Blank donor for Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci |
| 234142 | AAVS1-SA-CAG-Pa01-BFP | Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci |