Genetically encoded control of in vitro transcription-translation coupled DNA replication
Barthel S, Hoffmann-Becking M, Karimov IG, Erb TJ
bioRxiv 2025.07.08.663768; doi: 10.1101/2025.07.08.663768
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229030 | pTE5415 | Expression vector for 10xHis-phi29 DNA polymerase (wild-type): PT7-lacO-g10leader-p2 wt-T7 terminator, CamR |
| 229031 | pTE5422 | sfGFP-Pepper reporter: PT7-tetO-B0035-sfGFP-pepper-T7 terminator, AmpR |
| 229032 | pTE5425 | Expression vector for 10xHis-phi29 DNA polymerase (catalytically inactive mutant D249E): PT7-lacO-g10leader-p2 dead-T7 terminator, CamR |
| 229033 | pTE5426 | tetO-phi29 DNA polymerase wild-type reporter: PT7-tetO-B0035-p2 wt-T7 terminator, AmpR |
| 229034 | pTE5427 | tetO-phi29 DNA polymerase ‘dead’ reporter: PT7-tetO-B0035-p2 D249E-T7 terminator, AmpR |
| 229035 | pTE5428 | Expression vector for TetR: PT7-lacO-B0035-TetR-T7 terminator, AmpR |
| 229036 | pTE5429 | Expression vector for EilR: PT7-lacO-B0035-EilR-T7 terminator, AmpR |
| 229037 | pTE5430 | Azurite reporter: PT7-tetO-B0035-Azurite-T7 terminator, AmpR |
| 231064 | pTE5444 | Expression vector for 10x-phi29 DNA polymerase (PovSak): PT7-lacO-g10leader-10xHis-p2 povsak-T7 terminator, CamR |
| 231065 | pTE5448 | Expression vector for 10x-phi29 DNA polymerase (PovSakOk): PT7-lacO-g10leader-10xHis-p2 povsakok-T7 terminator, CamR |
| 231066 | pTE5451 | tetO-10xHis-phi29 DNA polymerase PovSak reporter: PT7-tetO-B0035-10xHis-p2 povsak-T7 terminator, AmpR |
| 231067 | pTE5452 | Inactive tetO-10xHis-phi29 DNA polymerase wild-type reporter: PT7-tetO-B0035-10xHis-p2 dead-T7 terminator, AmpR |