Yolanda Schaerli Lab: GRN_modeler: An Intuitive Tool for Constructing and Evaluating Gene Regulatory Networks and its Applications to Oscillators and a Light Biosensor
Holló G, Park JH, Boni E, Schaerli Y
bioRxiv 2024.12.18.629005
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229040 | pJP_Osc05 | Light inducible three-node circuit that can generate ring patterns in a growing bacterial colony upon blue light pulses. Heterologous expression of dCas9, Csy4, and VVD-AraC are necessary. |
| 230982 | pJP_Bla01 | Modified version of the pBLADE_ONLY_C (#168050). It expresses VVD-AraC, but the f1 ori was deleted and the chloramphenicol resistance gene was replaced by gentamicin resistance. |
| 230983 | pJP_Ctrl04 | pBAD promoter controls the expression of sfGFP. In the presence of VVD-AraC, blue light and L-arabinose can be used to activate sfGFP expression. |
| 230984 | pJP_1node | pBAD promoter controls the expression of sfGFP. In the presence of VVD-AraC, blue light and L-arabinose can be used to activate mCitrine expression. |