A tool for modeling gene regulatory networks (GRN_modeler) and its applications to synthetic biology.
Hollo G, Park JH, Boni E, Schaerli Y
Mol Syst Biol. 2025 Sep 29. doi: 10.1038/s44320-025-00148-8.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
229040 | pJP_Osc05 | Light inducible three-node circuit that can generate ring patterns in a growing bacterial colony upon blue light pulses. Heterologous expression of dCas9, Csy4, and VVD-AraC are necessary. |
230982 | pJP_Bla01 | Modified version of the pBLADE_ONLY_C (#168050). It expresses VVD-AraC, but the f1 ori was deleted and the chloramphenicol resistance gene was replaced by gentamicin resistance. |
230983 | pJP_Ctrl04 | pBAD promoter controls the expression of sfGFP. In the presence of VVD-AraC, blue light and L-arabinose can be used to activate sfGFP expression. |
230984 | pJP_1node | pBAD promoter controls the expression of sfGFP. In the presence of VVD-AraC, blue light and L-arabinose can be used to activate mCitrine expression. |