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A tool for modeling gene regulatory networks (GRN_modeler) and its applications to synthetic biology.

Hollo G, Park JH, Boni E, Schaerli Y
Mol Syst Biol. 2025 Sep 29. doi: 10.1038/s44320-025-00148-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
229040pJP_Osc05Light inducible three-node circuit that can generate ring patterns in a growing bacterial colony upon blue light pulses. Heterologous expression of dCas9, Csy4, and VVD-AraC are necessary.
230982pJP_Bla01Modified version of the pBLADE_ONLY_C (#168050). It expresses VVD-AraC, but the f1 ori was deleted and the chloramphenicol resistance gene was replaced by gentamicin resistance.
230983pJP_Ctrl04pBAD promoter controls the expression of sfGFP. In the presence of VVD-AraC, blue light and L-arabinose can be used to activate sfGFP expression.
230984pJP_1nodepBAD promoter controls the expression of sfGFP. In the presence of VVD-AraC, blue light and L-arabinose can be used to activate mCitrine expression.

Antibodies from Article