Cristina Cardoso Lab: MBD2 modulates heterchromatin architecture and composition

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
229559	pMBD2-1-GFP pc2067	Mammalian expression vector codes for MBD2(aa 1-152) fused c-terminal to GFP.
229560	pmMBD2.2-GFP pc2068	Mammalian expression vector codes for MBD2(aa 153-414) fused c-terminal to GFP.
229561	pmMBD2.4G pc2841	Mammalian expression vector codes for MBD2(aa 235-414) fused c-terminal to GFP.
229562	pmMBD2-6G pc2843	Mammalian expression vector codes for MBD2(aa 153-235) fused c-terminal to GFP.
229748	pTYB1-MBD1∆CxxC3 pc4783	Bacterial expression vector of MBD1 with a deletion of CXXC3 domain. C-terminal tagged to the intein.
229749	pTYB1-MBD3 pc4784	Bacterial expression vector of MBD3 C-terminal tagged to the intein.
229750	pTYB1-MBD4 pc4785	Bacterial expression vector of MBD4 C-terminal tagged to the intein.
229751	pTYB1-MBD2 pc4786	Bacterial expression vector of MBD2 C-terminal tagged to the intein.
229752	pTYB1-MBD2∆N(MBD2b) pc4787	Bacterial expression plasmid of MBD2∆N(MBD2b) lacking the N-terminus: aa 1-152. C-terminal tagged to intein.
229753	pTYB1-MBD2-N pc4789	Bacterial expression plasmid of MBD2-N (N-terminus of MBD2: aa 1-152). C-terminal tagged to intein.
229754	pTYB1-MBD2-MBDTRD pc4791	Bacterial expression vector of MBD2-MBDTRD domains (aa 153-235) C-terminal tagged to the intein.
229755	pTYB1-MBD2∆C pc4792	Bacterial expression plasmid of MBD2∆C lacking the C-terminus (a 236-414). C-terminal tagged to intein.
229756	pTYB1-MBD2-C pc4793	Bacterial expression plasmid of MBD2-C (C-terminus of MBD2: aa 236-414). C-terminal tagged to intein.
229757	pmMBD2∆C-G pc4794	Mammalian expression vector of MBD2. The Protein is lacking the C-terminus: aa 236-414. C-terminal tagged to GFP.
229758	pTYB1-MBD2∆GR pc5078	Bacterial expression plasmid of MBD2∆GR. The Protein is lacking the glycine/arginine rich domain: aa 75-96. C-terminal tagged to intein.
229759	pTYB1-MBD2∆C∆GR pc5079	Bacterial expression plasmid of MBD2∆C∆GR. MBD2 lacking the C-terminus of MBD2: aa 236-414 and the glycine/arginine rich domain: aa 75-96. C-terminal tagged to intein.
229760	pTYB1-MBD2a∆CC pc5081	Bacterial expression vector of MBD2a lacking the coiled coil domain: aa 363-396.
229761	pTYB1-MBD2-C∆CC pc5084	Bacterial expression plasmid of MBD2-C∆CC. C-terminus of MBD2 is lacking the coiled coil domain: aa 363-396. C-terminal tagged to intein.
229762	pmMBD2∆C∆GR-G pc5086	Mammalian expression plasmid of MBD2-∆C∆GR. N-terminus of MBD2 lacking the glycine/arginine rich domain: aa75-96. C-terminal tagged to GFP
229763	pmMBD2-N∆GR-G pc5087	Mammalian expression plasmid of MBD2-N∆GR. N-terminus of MBD2 lacking the glycine/arginine rich domain: aa75-96. C-terminal tagged to GFP.
229764	pmMBD2∆CC-G pc5088	Mammalian expression plasmid of MBD2∆CC. Protein is lacking the coiled coil domain: aa 363-396. C-terminal tagged to GFP.
229765	pmMBD2∆N∆CC-G pc5089	Mammalian expression plasmid of MBD2∆N∆CC. Protein is lacking the coiled coil domain: aa 363-396 and the N-terminus: aa 1-152. C-terminal tagged to GFP.
229766	pmMBD2-C∆CC-G pc5091	Mammalian expression plasmid of MBD2-C∆CC. C-terminus of MBD2 lacking the coiled coil domain: aa 363-396. C-terminal tagged to GFP.
230974	pTYB1-MBD2-N∆GR (pc5083)	Bacterial expression plasmid of MBD2-N∆GR. N-terminus of MBD2 lacking the glycine/arginine rich domain: aa75-96. C-terminal tagged to intein.