E2A, VA RNA I, and L4-22k adenoviral helper genes are sufficient for AAV production in HEK293 cells.
Doshi J, Couto E, Staiti J, Vandenberghe LH, Zabaleta N
Mol Ther Methods Clin Dev. 2024 Nov 13;32(4):101376. doi: 10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229478 | JD92 | CMV promoter driving expression of adenoviral E4orf6 gene |
| 229479 | JD94 | CMV promoter driving expression of adenoviral E2A gene |
| 229480 | JD80 | Adenoviral VA RNA I gene |
| 229481 | JD81 | Adenoviral VA RNA II gene |
| 229482 | JD73 | Adenoviral VA RNA I & II genes |
| 229483 | JD171 | Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genes |
| 229484 | JD226 | Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k genes |
| 229485 | JD150 | Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes |
| 229486 | JD180 | Adenoviral helper plasmid containing inducible E2A and L4-22k/33k genes |
| 229487 | JD141 | Ef1a promoter driving expression of adenoviral L4-22k/33k genes |
| 229488 | JD182 | Ef1a promoter driving expression of adenoviral L4-22k gene |
| 229489 | JD183 | Ef1a promoter driving expression of adenoviral L4-33k gene |
| 229490 | JD134 | Adenoviral E4 gene |
| 229491 | JD135 | Ef1a promoter driving expression of adenoviral 100k gene |
| 229492 | JD143 | CMV promoter driving expression of adenoviral E4orf6-6/7 gene |
| 229494 | JD108 | Adenoviral DeltaF6 plasmid variant devoid of fiber, U exon and pVIII sequences |
| 229495 | JD181 | Ef1a promoter driving expression of adenoviral 23k gene |
| 229496 | JD179 | Adenoviral helper plasmid containing inducible E2A and L4-22k/33k genes and constitutive VA RNA I gene |
| 229497 | JD136 | Adenoviral helper plasmid containing E2A and L4-22k/33k and VA RNA I genes |