The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-protein substrates in vitro.
Wang XS, Jiou J, Cerra A, Cobbold SA, Jochem M, Mak KHT, Corcilius L, Silke J, Payne RJ, Goddard-Borger ED, Komander D, Lechtenberg BC
Life Sci Alliance. 2025 Apr 1;8(6):e202503243. doi: 10.26508/lsa.202503243. Print 2025 Jun.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229539 | pOPINB-M1-di-Ub(G76V)-HOIL-1 | Constitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coli |