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Using Cupriavidus necator H16 to Provide a Roadmap for Increasing Electroporation Efficiency in Nonmodel Bacteria.

Vajente M, Clerici R, Ballerstedt H, Blank LM, Schmidt S
ACS Synth Biol. 2024 Oct 31. doi: 10.1021/acssynbio.4c00380. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
229553pMVRhaBroad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.

Antibodies from Article