De novo luciferases enable multiplexed bioluminescence imaging.
Chen JY-H, Shi Q, Peng X, Habimana JdD, Wang J, Sobolewski W, Yeh AH-W
Chem. 2025 Mar 13;11(3):102346. doi: 10.1016/j.chempr.2024.10.013. Epub 2024 Nov 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229942 | pBAD-luxNeon | Bacterial expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm) |
| 229943 | pBAD-luxGold | Bacterial expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm) |
| 229944 | pBAD-luxKOk | Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm) |
| 229945 | pBAD-luxOFP | Bacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588) |
| 229946 | pBAD-luxKate | Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638) |
| 229947 | pcDNA3-luxNeon | Mammalian expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm) |
| 229948 | pcDNA3-luxGold | Mammalian expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm) |
| 229949 | pcDNA3-luxKOk | Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm) |
| 229950 | pcDNA3-luxOFP | Mammalian expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588) |
| 229951 | pcDNA3-luxKate | Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638) |
| 229952 | pET29-neoLux1.2 | Bacterial expression for de novo luciferase, neoLux1.2 |