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De novo luciferases enable multiplexed bioluminescence imaging.

Chen JY-H, Shi Q, Peng X, Habimana JdD, Wang J, Sobolewski W, Yeh AH-W
Chem. 2025 Mar 13;11(3):102346. doi: 10.1016/j.chempr.2024.10.013. Epub 2024 Nov 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
229942pBAD-luxNeonBacterial expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)
229943pBAD-luxGoldBacterial expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)
229944pBAD-luxKOkBacterial expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)
229945pBAD-luxOFPBacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)
229946pBAD-luxKateBacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)
229947pcDNA3-luxNeonMammalian expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)
229948pcDNA3-luxGoldMammalian expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)
229949pcDNA3-luxKOkMammalian expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)
229950pcDNA3-luxOFPMammalian expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)
229951pcDNA3-luxKateMammalian expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)
229952pET29-neoLux1.2Bacterial expression for de novo luciferase, neoLux1.2

Antibodies from Article