Simplified MS2 phage-like particle production including a novel maturation protein internal fusion affinity tag.
Kline EC, Duong R, Wang Q, & Lutz BR.
Biotechnol Biotechnol Equip. 2025 Feb;39(1). doi: 10.1080/13102818.2025.2457691. Epub 2025 Feb 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229984 | pPLP1H-mCf | LIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification. |
| 229985 | pPLP2H-mCf | LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification. |
| 229986 | pPLP2S-mCf | LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification. |
| 229987 | pPLP3H-mCf | LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification. |
| 229988 | pPLP4HS-mCf | LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag and Twin-strep MP tag for AC purification. |