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Protocol for fast antibiotic resistance-based gene editing of mammalian cells with CRISPR-Cas9.

Adarska P, Fox E, Heyza J, Barnaba C, Schmidt J, Bottanelli F
STAR Protoc. 2025 Aug 4;6(3):103949. doi: 10.1016/j.xpro.2025.103949. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
230026loxP-G418-LoxP-TurboID-Rab11 HRHomology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

Antibodies from Article