Matthew Kennedy Lab: Genetically encoded intrabody probes for labeling and manipulating AMPA-type glutamate receptors

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
230055	pAAV-hSyn-GluA1-scFv-Halo	AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.
230056	pAAV-hSyn-GluA1-scFv-mNeon-KDEL	AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.
230057	pAAV-hSyn-GluA2-scFv-mNeon-KDEL	AAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.
230058	pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS	Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.
230059	pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS	Expresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.
230061	pCAG-GluA2-Fab-HC-Halo	Expresses heavy chain Fab fragment of anti-GluA2 fused to HaloTag. Can be used with light chain to produce Fab in HEK cells.
230063	pCAG-GluA2-Fab-LC	Expresses light chain Fab fragment of anti-GluA2. Can be used with heavy chain to produce Fab in HEK cells.
230065	pCAG-GluA1-scFV-Halo	Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.