Genetic- and culture-based tools for studying Bacteroides fragilis.
Schnizlein MK, Dubey AA, Fiebig A, Crosson S
Microbiol Resour Announc. 2025 May 8;14(5):e0000625. doi: 10.1128/mra.00006-25. Epub 2025 Mar 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 230064 | pMS01 | For allelic exchange into Bacteroides fragilis (pLGB13-based); ermC, aTC-inducible counterselection with ssbfe1; R6K ori, RP4 oriT |
| 230066 | pMS02 | Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT |
| 230068 | pMS04 | Inducible Promoter (anhydrotetracycline-induced, tetO/tetR system), integrates via IntN1; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT |
| 230069 | pMS05 | Inducible Promoter (anhydrotetracycline-induced, tetO/tetR system), replicates via RepA; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT |
| 230071 | pMS06 | Inducible Promoter (rhamnose-induced, BT3763/rhaR system), integrates via IntN1; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT |
| 230073 | pMS09 | Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; N-/C-terminal iLOV fusion, ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT |
| 230074 | pMS10 | Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; N-/C-terminal mNeonGreen fusion, ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT |
| 230937 | pMS03 | Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), replicates via RepA; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT |