AAVone: A cost-effective, single-plasmid solution for efficient AAV production with reduced DNA impurities.
Yang R, Tran NT, Chen T, Cui M, Wang Y, Sharma T, Liu Y, Zhang J, Yuan X, Zhang D, Chen C, Shi Z, Wang L, Dai Y, Zaidi H, Liang J, Chen M, Jaijyan D, Hu H, Wang B, Xu C, Hu W, Gao G, Yu D, Tai PWL, Wang Q
Mol Ther Nucleic Acids. 2025 May 14;36(2):102563. doi: 10.1016/j.omtn.2025.102563. eCollection 2025 Jun 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 230928 | pAAVone-AAV9-CMV-eGFP | pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system. |
| 230929 | pAAVone-AAV-PHP.eB-CMV-eGFP | pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV-PHP.eB serotype, utilizing the AAVone single-plasmid AAV production system. |
| 230930 | pAAVone-AAV2-CMV-eGFP | pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV2 serotype, utilizing the AAVone single-plasmid AAV production system. |
| 230931 | pAAVdual-CMV-eGFP | pAAVdual plasmid is used to package AAV-CMV-eGFP viruses with the AAVdual system, which integrates the mini-pHelper-1.0 (providing E2A, E4orf6, and VA RNA) with an AAV expression cassette containing eGFP driven by the CMV promoter. |
| 230932 | pAAVtri-CMV-eGFP | Used for producing AAV-CMV-eGFP viruses with the AAVtri triple-plasmid system. This system combines the use of the mini-pHelper-1.0 and AAV helper plasmids carrying different AAV serotypes. |
| 230933 | mini-pHelper-1.0 | The mini verison (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production |