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AAVone: A cost-effective, single-plasmid solution for efficient AAV production with reduced DNA impurities.

Yang R, Tran NT, Chen T, Cui M, Wang Y, Sharma T, Liu Y, Zhang J, Yuan X, Zhang D, Chen C, Shi Z, Wang L, Dai Y, Zaidi H, Liang J, Chen M, Jaijyan D, Hu H, Wang B, Xu C, Hu W, Gao G, Yu D, Tai PWL, Wang Q
Mol Ther Nucleic Acids. 2025 May 14;36(2):102563. doi: 10.1016/j.omtn.2025.102563. eCollection 2025 Jun 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
230928pAAVone-AAV9-CMV-eGFPpAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.
230929pAAVone-AAV-PHP.eB-CMV-eGFPpAAVone plasmid is used to package AAV-CMV-eGFP with an AAV-PHP.eB serotype, utilizing the AAVone single-plasmid AAV production system.
230930pAAVone-AAV2-CMV-eGFPpAAVone plasmid is used to package AAV-CMV-eGFP with an AAV2 serotype, utilizing the AAVone single-plasmid AAV production system.
230931pAAVdual-CMV-eGFPpAAVdual plasmid is used to package AAV-CMV-eGFP viruses with the AAVdual system, which integrates the mini-pHelper-1.0 (providing E2A, E4orf6, and VA RNA) with an AAV expression cassette containing eGFP driven by the CMV promoter.
230932pAAVtri-CMV-eGFPUsed for producing AAV-CMV-eGFP viruses with the AAVtri triple-plasmid system. This system combines the use of the mini-pHelper-1.0 and AAV helper plasmids carrying different AAV serotypes.
230933mini-pHelper-1.0The mini verison (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production

Antibodies from Article