A Cellular Mechanism to Detect and Alleviate Reductive Stress.
Manford AG, Rodriguez-Perez F, Shih KY, Shi Z, Berdan CA, Choe M, Titov DV, Nomura DK, Rape M
Cell. 2020 Oct 1;183(1):46-61.e21. doi: 10.1016/j.cell.2020.08.034. Epub 2020 Sep 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 230993 | pCS2+ 3xFLAG-FEM1B WT | Transient overexpression of mouse FEM1B in mammalian cells |
| 230994 | pCS2+ 3xFLAG-FEM1B L597A | Transient overexpression of catalytic null mouse FEM1B in mammalian cells. Mutation abolishes interaction with CUL2. |
| 230995 | pCS2+ 3xFLAG-FEM1B C186S | Transient overexpression of mouse FEM1B in mammalian cells. Mutation abolishes substrate binding. |
| 230996 | pCS2+ GFP-2xESP3I-IRES-mCherry | Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression. |
| 230999 | pRSFDuet-1 ElonginB, ElonginC | Dual expression vector containing mouse ElonginB and ElonginC for E.coli protein purification |
| 231000 | pFastBac Dual His6-TEV-CUL2, Rbx1 | Dual expression vector containing mouse CUL2 and Rbx1 for protein puritification from SF9 insect cells |