High-Complexity Barcoded Rabies Virus for Scalable Circuit Mapping Using Single-Cell and Single-Nucleus Sequencing.
Shin D, Urbanek ME, Larson HH, Moussa AJ, Lee KY, Baker DL, Standen-Bloom E, Ramachandran S, Bogdanoff D, Cadwell CR, Nowakowski TJ
bioRxiv [Preprint]. 2024 Dec 11:2024.10.01.616167. doi: 10.1101/2024.10.01.616167.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227671 | pSADB19-dTomato Barcode Library | RVdG genome plasmids that carry an error-correctable barcode compatible with 3’ capture single cell or single nuclei RNA sequencing readout. For the production of SADB19 strain G-deleted rabies virus. |
| 227672 | pCVSN2c-MCP-oScarlet-KASH-6xMS2 Barcode Library | RVdG genome plasmids that carry an error-correctable barcode compatible with 3’ capture single cell or single nuclei RNA sequencing readout. For the production of CVS N2c strain G-deleted rabies virus |
| 231009 | pSico-syn-GFP-TVA-oG-WPRE-bGHpA | Lentiviral helper virus for G-deleted rabies tracing |
| 231010 | pSico-syn-mGreenLantern:KASH-TVA-oG-6xMBS-WPRE-bGHpA | Lentiviral helper virus for G-deleted rabies tracing. mGreenLantern:KASH fluorescence is not visible without immunostaining. |
| 231011 | CVS-N2c-RVdG-MCP:oScarlet:KASH-6xMBS | CVS N2c RVdG genome plasmid. Utilizes MS2 tagging to deliver the MCP:oScarlet:KASH transcript to the outer nuclear membrane. Insert high complexity barcode between SacII RE sites distal to 6xMBS site. |
| 231013 | pSico-U6-tornado:6xMBS:8G8ApA-syn-GFP-TVA-oG-WPRE-bGHpA | Lentiviral helper virus for G-deleted rabies tracing, with U6-driven transcript amenable for nuclear capture in the presence of RVdG expressing MS2-KASH. |
| 231014 | CVS-N2c-RVdG-MCP:oScarlet-KASH_NoMBS | CVS N2c RVdG genome plasmid, utilized to generate negative control virus to benchmark the utility of MS2 tagging for capture of barcodes using snRNA-seq. |