Bottom-up reconstruction of functional Death Domain Signalosomes reveals a requirement for polymer stability and avidity
Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E, Taylor MJ
bioRxiv 2024.05.08.593169
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233919 | pHRSV_CHARMS-TIR | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS): CHARMS-TIR |
| 233920 | pHRSV_CHARMS | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS |
| 233921 | pHRSV_CHARMS-3xAla | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xAla (mutated TRAF6 binding site) |
| 233922 | pHRSV_CHARMS-DHF | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-DHF (Designed Helical Filament) |
| 233923 | pHRSV_CHARMS-1x | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1x which contains only 1 TRAF6 binding motif |
| 233924 | pHRSV_CHARMS-1xA | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1xA which contains 1 TRAF6 binding motif with a alanine mutation |
| 233925 | pHRSV_CHARMS-3x | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 3 TRAF6 binding motifs |
| 233926 | pHRSV_CHARMS-3xA | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xA which contains 3 TRAF6 binding motif with a alanine mutation |
| 233927 | pHRSV_CHARMS-5x | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 5 TRAF6 binding motifs |
| 233928 | pHRSV_CHARMS-5xA | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5xA which contains only 5 TRAF6 binding motifs with a alanine mutation |
| 233929 | pHRSV_CHARMS-S34Y | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-S34Y which contains a mutation in the death domain that disrupt oligomer formation |
| 233931 | pHRSV_CHARMS_bDD | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-dDD contains the bacterial death domain from Nostoc. |
| 233932 | pHRSV_CHARMS-11xTA | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-11xTAcontains 11 TA repeats in place of the death domain |
| 233933 | pHRSV_CHARMS-15xTA | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-15xTAcontains 15 TA repeats in place of the death domain |
| 233934 | pHRSV_CHARMS-17xTA | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-17xTAcontains 17 TA repeats in place of the death domain |
| 233935 | pHRSV_CHARMS-20xTA | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-10xTAcontains 20 TA repeats in place of the death domain |
| 233936 | pHRSV_CHARMS-17xTA-3xAla | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-17xTA with alanine mutation in the TRAF6 binding motif |
| 233937 | pHRSV_CHARMS-20xTA-3xAla | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-20xTA with alanine mutation in the TRAF6 binding motif |
| 233938 | pHRSV_CHARMS-TIR-5xT6BMs | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-TIR with 5 TRAF6 binding motifs |
| 233939 | pHRSV_CHARMS-bDD-5xT6BMs | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-bDD with 5 TRAF6 binding motifs |
| 233940 | pHRSV_CHARMS-V43D/E52K/R62E | Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS three mutations in the death domain that disrupt oligomer formation |