RNA-stabilized coat proteins for sensitive and simultaneous imaging of distinct single mRNAs in live cells.
Kuffner CJ, Marzilli AM, Ngo JT
Nat Methods. 2025 Sep 22. doi: 10.1038/s41592-025-02782-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231089 | pcDNA3-mNG-IRES-mScarlet-dMCP | Bicistronic construct encoding mNG in combination with mScarlet-dMCP via an EMCV-IRES sequence. |
| 231090 | pcDNA3-mNG-IRES-mScarlet-dPCP | Bicistronic construct encoding mNG in combination with mScarlet-dPCP via an EMCV-IRES sequence. |
| 231091 | minCMV-1xmNG-dMCP | Encodes a 1xmNeonGreen-dMCP under control of a weak promoter (minCMV). |
| 231092 | minCMV-1xmNG-dPCP | Encodes a 1xmNeonGreen-dPCP under control of a weak promoter (minCMV). |
| 231093 | UBC-4xmNG-dMCP | Encodes a 4xmNeonGreen-dMCP under control of a weak promoter (UBC). |