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BEVA2.0: modular assembly of golden gate-compatible vectors with expanded utility for genetic engineering.

Geddes BA, Williamson R, Schumacher J, Ardi A, Levin G, Cervenka E, Huang R, diCenzo G
Can J Microbiol. 2025 Jan 1;71:1-13. doi: 10.1139/cjm-2024-0246. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
231305pNDGG021Vector part for BEVA; BEVA2.0 Position 1 Level 1 BsaI cloning site without T0, AmpR
231306pNDGG022Vector part for BEVA; BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR
231307pNDGG001Vector part for BEVA; BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.
231308pNDGG002Vector part for BEVA; BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR
231309pNDGG005Vector part for BEVA; BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.
231310pNDGG006Vector part for BEVA; BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.
231311pNDGG007Vector part for BEVA; BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
231312pNDGG008Vector part for BEVA; BEVA2.0 Position 4 sacB sucrose counterselection module, AmpR.
231313pQGG001Vector part for BEVA; BEVA2.0 Position 4 I-SceI recognition/cut site, SpR.
231314pNDGG009Vector part for BEVA; BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.
231315pNDGG010Vector part for BEVA; BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.
231316pNDGG003BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Tc-par-ELT4; GG assembly from pOGG010, pOGG012, pOGG014, pOGG042, and pOGG004, TcR
231317pNDGG004BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Tc-par-ELT4; GG assembly from pOGG011, pOGG012, pOGG014, pOGG042, and pOGG004, TcR
231318pNDGG049BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. -pBBR1-Gm-par-ELT4; GG assembly from pOGG004, pOGG009, pOGG011, pOGG012, and pOGG014, GmR
231319pNDGG050BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Gm-par-ELT4; GG assembly from pOGG004, pOGG009, pOGG010, pOGG012, and pOGG014, GmR
231320pNDGG051BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Sp-par-ELT4; GG assembly from pOGG004, pNDGG002, pOGG011, pOGG012, and pOGG014, SpR
231321pNDGG052BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Sp-par-ELT4; GG assembly from pOGG004, pNDGG002, pOGG010, pOGG012, and pOGG014, SpR
231322pNDGG053BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG011, pOGG012, and pOGG014, NmR/KmR
231323pNDGG054BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG010, pOGG012, and pOGG014, NmR/KmR
231324pNDGG056BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RSF1010-Gm-par-ELT4; GG assembly from pGQ0015, pOGG009, pNDGG005, pOGG012, and pOGG014, GmR
231325pNDGG059BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RSF1010-Tc-par-ELT4; GG assembly from pOGG004, pOGG042, pNDGG005, pOGG012, and pOGG014, TcR
231326pNDGG058BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RSF1010-Sp-par-ELT4; GG assembly from pGQ0015, pNDGG002, pNDGG005, pOGG012, and pOGG014, SpR
231327pNDGG057BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RSF1010-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pNDGG005, pOGG012, and pOGG014, NmR/KmR
231328pNDGG070BEVA Golden Gate cloning vector; BEVA2.0 Sucrose curable broad host range plasmid . L1-pBBR1-Sp-sacB-ELT4; GG assembly from pOGG004, pNDGG002, pOGG011, pNDGG008, and pOGG014, SpR
231329pQGG002BEVA Golden Gate cloning vector; BEVA2.0 Narrow host range plasmid with I-SceI counterselectable site. L1-P15A-Nm-ISceI; GG assembly from pOGG004, pNDGG001, pNDGG006, pGQ0023, pOGG014. KmR/NmR.
231330pQGG003BEVA Golden Gate cloning vector; BEVA2.0 Narrow host range plasmid with I-SceI counterselectable site for BpiI cloning; KmR/NmR.
231331pQGG004BEVA Golden Gate cloning vector; BEVA2.0 Narrow host range plasmid with I-SceI counterselectable site. L1-P15A-Gm-ISceI-ELT4; GG assembly from pOGG004, pOGG009, pNDGG006, pGQ0023, pOGG014. GmR.
231332pQGG005BEVA Golden Gate cloning vector; BEVA2.0 Narrow host range plasmid with I-SceI-ELT4 counterselectable site for BpiI cloning. L2-P15A-Gm-ISceI-ELT4; GG assembly from pOGG006, pOGG009, pNDGG006, pGQ0023, pOGG014. GmR.
231333pNDGG012BEVA Golden Gate cloning vector; Narrow host range plasmid with FRT site. L1-pMB1-Gm-FRT-ELT4; GG assembly from pNDGG021, pOGG009, pNDGG007, pNDGG009, pOGG014. GmR.
231334pNDGG013BEVA Golden Gate cloning vector; Narrow host range plasmid with FRT site. L1-p15A-Gm-FRT-ELT4; GG assembly from pNDGG021, pOGG009, pNDGG006, pNDGG009, pOGG014. NmR.
231335pNDGG014BEVA Golden Gate cloning vector; Narrow host range plasmid. L1-pMB1-Gm-ELT3; pNDGG021, pOGG009, pNDGG007, pOGG013. GmR.
231336pNDGG015BEVA Golden Gate cloning vector; Narrow host range plasmid. L1-p15A-Gm-ELT3; pNDGG021, pOGG009, pNDGG006, pOGG013. NmR.
231337pNDGG037Terminator; BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.
231338pNDGG038Terminator; BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.
231339pNDGG039Terminator; BEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.

Antibodies from Article