Fine-Tuning Genetic Circuits via Host Context and RBS Modulation.

Chan DTC, Winter L, Bjerg J, Krsmanovic S, Baldwin GS, Bernstein HC
ACS Synth Biol. 2025 Jan 17;14(1):193-205. doi: 10.1021/acssynbio.4c00551. Epub 2025 Jan 4. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
231615	pVCS1	Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS1 at R2 site.
231616	pVCS2	Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS2 at R2 site.
231617	pVCS3	Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS3 at R2 site.
231618	pVCS4	Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS1 at R2 site.
231619	pVCS5	Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS2 at R2 site.
231620	pVCS6	Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS3 at R2 site.
231621	pVCS7	Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS1 at R2 site.
231622	pVCS8	Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS2 at R2 site.
231623	pVCS9	Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS3 at R2 site.