RNA-coupled CRISPR Screens Reveal ZNF207 as a Regulator of LMNA Aberrant Splicing in Progeria.
Behera AK, Kim JJ, Kordale S, Damodaran AP, Kumari B, Vidak S, Dickson E, Xiao MS, Duncan G, Andresson T, Misteli T, Gonatopoulos-Pournatzis T
bioRxiv [Preprint]. 2025 Apr 26:2025.04.25.648738. doi: 10.1101/2025.04.25.648738.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232068 | CRASP-Seq CBC Backbone | The CRASP-Seq backbone is designed to accommodate the cloning of diverse gRNA libraries. It includes a cell barcode (CBC), which enhances its functionality by enabling library preparation. |
| 232069 | CRASP-Seq Gene KO v1 empty | Flexible CRASP-Seq platform for studying splicing regulation. Used to study how protein coding genes impact splicing of minigene reporters. |
| 232070 | CRASP-Seq BE Tiling v1 empty | Tiling guides designed to target 39 splicing-related genes, along with several thousand control gRNAs. Optimized for CRASP-Seq experiments combined with high-throughput base editing mutagenesis. |