RNA-coupled CRISPR Screens Reveal ZNF207 as a Regulator of LMNA Aberrant Splicing in Progeria.
Behera AK, Kim JJ, Kordale S, Damodaran AP, Kumari B, Vidak S, Dickson E, Xiao MS, Duncan G, Andresson T, Misteli T, Gonatopoulos-Pournatzis T
bioRxiv [Preprint]. 2025 Apr 26:2025.04.25.648738. doi: 10.1101/2025.04.25.648738.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231928 | pLCHKO-CRASP | Empty backbone for CRASP-Seq experiments |
| 231929 | pcDNA5-ZNF207-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207-3xFLAG |
| 231930 | pcDNA5-ZNF207_∆ZnF_-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆ZnF_-3xFLAG |
| 231931 | pcDNA5-ZNF207_∆Hel_-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆Hel_-3xFLAG |
| 231932 | pcDNA5-ZNF207_∆QPG_-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆QPG_-3xFLAG |
| 231933 | pcDNA5-ZNF207_∆RNA1-2_-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆RNA1-2_-3xFLAG |
| 231934 | pcDNA5-ZNF207_∆polar_-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆polar_3xFLAG |
| 231935 | pcDNA5-ZNF207_∆GLEBS_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆GLEBS_3xFLAG |
| 231936 | pcDNA5-ZNF207_∆C-term_-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆C-term_3xFLAG |
| 231937 | pcDNA5-ZNF207_∆RNA3_-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆RNA3_3xFLAG |
| 231938 | pcDNA5-ZNF207-miniTurbo | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207-miniTurbo |
| 231939 | pLenti-ZNF207-3xFLAG | Vector for generating stable lines allowing expression of ZNF207-3xFLAG |
| 232068 | CRASP-Seq CBC Backbone | The CRASP-Seq backbone is designed to accommodate the cloning of diverse gRNA libraries. It includes a cell barcode (CBC), which enhances its functionality by enabling library preparation. |
| 232069 | CRASP-Seq Gene KO v1 empty | Knockout library for use in CRASP-Seq experiments. Provides a flexible platform for studying splicing regulation. |
| 232070 | CRASP-Seq BE Tiling v1 empty | Tiling library to conduct CRASP-Seq experiments combined with high-throughput base editing for precise and scalable investigations into protein regions that affect splicing regulation. |
| 247229 | pcDNA5-ZNF207-ZnF_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-ZnF_3xFLAG |
| 247230 | pcDNA5-ZNF207-ZnF+Hel_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-ZnF+Hel_3xFLAG |
| 247231 | pcDNA5-ZNF207-ZnF+Hel_K42E_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-ZnF+Hel_K42E_3xFLAG |
| 247232 | pcDNA5-ZNF207-ZnF+Hel_K42M_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-ZnF+Hel_K42M_3xFLAG |
| 247233 | pcDNA5-ZNF207-ZnF+Hel_K42R_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-ZnF+Hel_K42R_3xFLAG |
| 247234 | pcDNA5-ZNF207-K42E_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-K42E_3xFLAG |
| 247235 | pcDNA5-ZNF207-K42M_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-K42M_3xFLAG |
| 247236 | pcDNA5-ZNF207-K42R_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-K42R_3xFLAG |