RNA-coupled CRISPR screens reveal ZNF207 as a regulator of LMNA aberrant splicing in progeria.
Behera AK, Kim JJ, Kordale S, Pekovic F, Damodaran AP, Kumari B, Vidak S, Dickson E, Xiao MS, Duncan G, Andresson T, Misteli T, Valkov E, Gonatopoulos-Pournatzis T
Mol Cell. 2026 Jan 8;86(1):41-59.e15. doi: 10.1016/j.molcel.2025.12.003. Epub 2025 Dec 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231928 | pLCHKO-CRASP | Empty backbone for CRASP-Seq experiments |
| 231929 | pcDNA5-ZNF207-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207-3xFLAG |
| 231930 | pcDNA5-ZNF207_∆ZnF_-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆ZnF_-3xFLAG |
| 231931 | pcDNA5-ZNF207_∆Hel_-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆Hel_-3xFLAG |
| 231932 | pcDNA5-ZNF207_∆QPG_-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆QPG_-3xFLAG |
| 231933 | pcDNA5-ZNF207_∆RNA1-2_-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆RNA1-2_-3xFLAG |
| 231934 | pcDNA5-ZNF207_∆polar_-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆polar_3xFLAG |
| 231935 | pcDNA5-ZNF207_∆GLEBS_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆GLEBS_3xFLAG |
| 231936 | pcDNA5-ZNF207_∆C-term_-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆C-term_3xFLAG |
| 231937 | pcDNA5-ZNF207_∆RNA3_-3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆RNA3_3xFLAG |
| 231938 | pcDNA5-ZNF207-miniTurbo | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207-miniTurbo |
| 231939 | pLenti-ZNF207-3xFLAG | Vector for generating stable lines allowing expression of ZNF207-3xFLAG |
| 232068 | CRASP-Seq CBC Backbone | The CRASP-Seq backbone is designed to accommodate the cloning of diverse gRNA libraries. It includes a cell barcode (CBC), which enhances its functionality by enabling library preparation. |
| 232069 | CRASP-Seq Gene KO v1 empty | Knockout library for use in CRASP-Seq experiments. Provides a flexible platform for studying splicing regulation. |
| 232070 | CRASP-Seq BE Tiling v1 empty | Tiling library to conduct CRASP-Seq experiments combined with high-throughput base editing for precise and scalable investigations into protein regions that affect splicing regulation. |
| 247229 | pcDNA5-ZNF207-ZnF_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-ZnF_3xFLAG |
| 247230 | pcDNA5-ZNF207-ZnF+Hel_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-ZnF+Hel_3xFLAG |
| 247231 | pcDNA5-ZNF207-ZnF+Hel_K42E_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-ZnF+Hel_K42E_3xFLAG |
| 247232 | pcDNA5-ZNF207-ZnF+Hel_K42M_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-ZnF+Hel_K42M_3xFLAG |
| 247233 | pcDNA5-ZNF207-ZnF+Hel_K42R_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-ZnF+Hel_K42R_3xFLAG |
| 247234 | pcDNA5-ZNF207-K42E_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-K42E_3xFLAG |
| 247235 | pcDNA5-ZNF207-K42M_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-K42M_3xFLAG |
| 247236 | pcDNA5-ZNF207-K42R_3xFLAG | Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-K42R_3xFLAG |