PAF1C-mediated activation of CDK12/13 kinase activity is critical for CTD phosphorylation and transcript elongation
Martinez DL, Todorovski I, Gonzalez MN, Rusimbi C, Blears D, Khallou N, Han Z, Dirac-Svejstrup B, Svejstrup JQ
Molecular Cell. 01 Mar 2025. doi: 10.1016/j.molcel.2025.04.012
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231716 | pLIB-FLAG(N)-CDK12 | Protein expression in insect cells |
| 231717 | pLIB-FLAG(N)-CDK13 | Protein expression in insect cells |
| 231718 | pLIB-Cyclin K | Protein expression in insect cells |
| 231719 | pLIB-FLAG(N)-CDK9 | Protein expression in insect cells |
| 231720 | pLIB-Cyclin T1 | Protein expression in insect cells |
| 231721 | pLIB-FLAG-GST(N)-RPB1-CTD(1586-1971) | Protein expression in insect cells |
| 231722 | pLIB-FLAG(N)-RPB1 | Protein expression in insect cells |
| 231723 | pLIB-His(N)-SSU72 | Protein expression in insect cells |
| 231724 | pLIB-Strep(II)(N)-PIN1 | Protein expression in insect cells |
| 231725 | pLIB-Strep(II)(C)-CTR9 | Protein expression in insect cells |
| 231726 | pLIB-FLAG(N)-CDC73 | Protein expression in insect cells |
| 231727 | pLIB-LEO1 | Protein expression in insect cells |
| 231728 | pLIB-PAF1 | Protein expression in insect cells |
| 231729 | pLIB-WDR61 | Protein expression in insect cells |
| 231730 | pBIG1e-CDK12/Cyclin K | Protein expression in insect cells |
| 231731 | pBIG1e-CDK13/Cyclin K | Protein expression in insect cells |
| 231732 | pBIG1e-CDK9/Cyclin T1 | Protein expression in insect cells |
| 231733 | pBIG1a-PAF1C(CTR9/CDC73/PAF1/LEO1/WDR61) | Protein expression in insect cells |
| 231734 | pLIB-FLAG(N)-CDK12deltaNTD(700-1490) | Protein expression in insect cells |
| 231735 | pLIB-FLAG(N)-CDK12deltaCTD(1-1082) | Protein expression in insect cells |
| 231736 | pLIB-FLAG(N)-CDK12-KiD(700-1082) | Protein expression in insect cells |
| 231737 | pLIB-FLAG(N)-CDK12deltaRS(delta104-380) | Protein expression in insect cells |
| 231738 | pLIB-FLAG-GST(N)-CDK12RS(104-380) | Protein expression in insect cells |
| 231739 | pLIB-FLAG(N)-CDC73deltaKIM(delta290-324) | Protein expression in insect cells |
| 231740 | pLIB-FLAG(N)-CDC73-YY/AA(Y290A, Y293A) | Protein expression in insect cells |
| 231741 | pBIG1e-CDK12deltaNTD /Cyclin K | Protein expression in insect cells |
| 231742 | pBIG1e-CDK12deltaCTD/Cyclin K | Protein expression in insect cells |
| 231743 | pBIG1e-CDK12-KiD/Cyclin K | Protein expression in insect cells |
| 231744 | pBIG1e-CDK12deltaRS/Cyclin K | Protein expression in insect cells |
| 231745 | pBIG1a-PAF1CdeltaWDR61 | Protein expression in insect cells |
| 231746 | pBIG1a-PAF1CdeltaCDC73 | Protein expression in insect cells |
| 231747 | pBIG1a-PAF1CdeltaLEO1 | Protein expression in insect cells |
| 231748 | pBIG1a-PAF1C-CDC73deltaKIM | Protein expression in insect cells |
| 231749 | pBIG1a-PAF1C-CDC73-YY/AA | Protein expression in insect cells |
| 231750 | pFRT-FLAG-HA-(N)-CDK12 | Expression in Flp-IN human cells |
| 231751 | pFRT-TO-FLAG(N)-CDC73 | Expression in Flp-IN human cells |
| 231752 | pFRT-TO-FLAG(N)-CDC73deltaKIM | Expression in Flp-IN human cells |
| 231753 | pFRT-TO-FLAG(N)-CDC73-YY/AA | Expression in Flp-IN human cells |