CRISPRi-ART enables functional genomics of diverse bacteriophages using RNA-binding dCas13d.
Adler BA, Al-Shimary MJ, Patel JR, Armbruster EG, Colognori D, Charles EJ, Miller KV, Lahiri A, Cui ML, Oromi-Bosch A, Voelker A, Trinidad M, Lee J, Beurnier S, Boger R, Nomburg J, Barrangou R, Mutalik VK, Schoeniger JS, Pogliano JA, Savage DF, Doudna JA, Cress BF
Nat Microbiol. 2025 Mar;10(3):694-709. doi: 10.1038/s41564-025-01935-7. Epub 2025 Feb 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231992 | pBFC0984 | CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site |
| 231993 | pBFC0993 | CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer |
| 231994 | pBFC1171 | CRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning site |