Improved vectors for retron-mediated CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.

Stuecker TN, Hood SE, Molina Pineda J, Lenaduwe S, Winter J, Sadhu MJ, Lewis JA
G3 (Bethesda). 2025 Aug 4:jkaf175. doi: 10.1093/g3journal/jkaf175. PubMed Article

These materials are available to order together as the Expanded CRISPEY Kit.

Plasmids from Article

ID	Plasmid	Purpose
232090	pCas9-EcRT-GAL-Nat	Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
232091	pCas9-EcRT-GAL-Hyg	Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
232092	pCas9-EcRT-GAL-Kan	Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
232093	pCas9-EcRT-GAL-HIS3	Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
232094	pCas9-EcRT-Z3-Nat	Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.
232095	pCas9-EcRT-Z3-Hyg	Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.
232096	pCas9-EcRT-Z3-Kan	Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.
232097	pCRISPEY-GAL-Nat	Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selection
232098	pCRISPEY-GAL-Hyg	Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection
232099	pCRISPEY-GAL-Kan	Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection
232100	pCRISPEY-Z3-Nat	Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.
232101	pCRISPEY-Z3-Hyg	Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.
232102	pCRISPEY-Z3-Kan	Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.
232103	pCRISPEY-GAL-Nat-ADE2	Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
232104	pCRISPEY-GAL-Hyg-ADE2	Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
232105	pCRISPEY-GAL-Kan-ADE2	Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
232106	pCRISPEY-Z3-Nat-ADE2	Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
232107	pCRISPEY-Z3-Hyg-ADE2	Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.