Seeing is believing: Genome editing made easy with RUBY for educational purposes.

Sexton WK, Cheng Y, Yang B, Ullah H, Qi Y, Xiao S
Plant Physiol. 2025 Jul 3;198(3):kiaf265. doi: 10.1093/plphys/kiaf265. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
232175	RUBYm2	Introduce a C-to-T mutation to generate an early stop codon (Q139 to stop), thereby interrupting RUBY expression
232176	RUBY-ABE#2	T-DNA vector with ecTadA8e-zCas9D10A and corresponding sgRNA to correct RUBYm2 and recover a vivid red color visible to the naked eyes.