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Accelerating protein design by scaling experimental characterization

Qian J, Milles LF, Wicky BIM, Motmaen A, Li X, Kibler RD, Stewart L, Baker D
bioRxiv 2025.08.05.668824; doi: https://doi.org/10.1101/2025.08.05.668824 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
232210cwby0001GGA cloning M-AGGA-promoter-ccdb-TTCC-SNAC-HIS
232211cwby0002GGA cloning MSG-AGGA-promoter-ccdb-TTCC-HIS
232212cwby0003GGA cloning sfGFP-AGGA-ccdb-TTCC-HIS
232213cwby0004GGA cloning cwby template vector pet29-ATG-PaQCI-TAAT_GA
232214cwby0005GGA cloning MS-his-AGGA-promoter-ccdb-TTCC
232215cwby0006GGA cloning TATG-promotor-ccdb-TAAT
232216cwby0007GGA cloning M-AGGA-promoter-ccdb-TTCC-Avitag-HIS
232217cwby0008GGA cloning M-AGGA-promoter-ccdb-TTCC-HIS-SpyTag003
232218cwby0009GGA cloning his-mScarletI-AGGA-promoter-ccdb-TTCC-his
232219cwby0010GGA cloning his-TagGFP2-AGGA-promoter-ccdb-TTCC
232220cwby0011GGA cloning his-mTagBFP2-AGGA-promoter-ccdb-TTCC
232221cwby0012GGA cloning his-mscarletI3-AGGA-promoter-ccdb-TTCC
232222cwby0013GGA cloning his-muGFP-AGGA-promoter-ccdb-TTCC
232223cwby0014GGA cloning his-mBaoJin-AGGA-promoter-ccdb-TTCC
232224cwby0015GGA cloning his-mhfYFP-AGGA-promoter-ccdb-TTCC
247377cwby0027GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC1_008-SNAC-6xhis
247378cwby0028GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC2_059-SNAC-6xhis
247380cwby0030GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC2_063-SNAC-6xhis
247381cwby0031GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC2_064-SNAC-6xhis
247382cwby0032GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC2_065-SNAC-6xhis
247383cwby0033GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC2_067-SNAC-6xhis
247384cwby0034GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC2_068-SNAC-6xhis
247385cwby0035GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC3_104-SNAC-6xhis
247386cwby0036GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC3_109-SNAC-6xhis
247387cwby0037GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC3_110-SNAC-6xhis
247388cwby0038GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC3_114-SNAC-6xhis
247389cwby0039GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC3_118-SNAC-6xhis
247390cwby0040GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC3_919-SNAC-6xhis
247391cwby0041GGA cloning AGGA-promoter-ccdb-TTCC-GS-SB175-SNAC-6xhis
247392cwby0042GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC4_135-SNAC-6xhis
247394cwby0044GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC4_140-SNAC-6xhis
247395cwby0045GGA cloning AGGA-promoter-ccdb-TTCC-GS-HALC5_169-SNAC-6xhis
247397cwby0047GGA cloning AGGA-HALC1_004-GS-promoter-ccdb-TTCC-SNAC-6xhis
247398cwby0048GGA cloning AGGA-HALC1_008-GS-promoter-ccdb-TTCC-SNAC-6xhis
247399cwby0049GGA cloning AGGA-HALC2_059-GS-promoter-ccdb-TTCC-SNAC-6xhis
247400cwby0050GGA cloning AGGA-HALC2_062-GS-promoter-ccdb-TTCC-SNAC-6xhis
247401cwby0051GGA cloning AGGA-HALC2_063-GS-promoter-ccdb-TTCC-SNAC-6xhis
247402cwby0052GGA cloning AGGA-HALC2_064-GS-promoter-ccdb-TTCC-SNAC-6xhis
247403cwby0053GGA cloning AGGA-HALC2_065-GS-promoter-ccdb-TTCC-SNAC-6xhis
247404cwby0054GGA cloning AGGA-HALC2_067-GS-promoter-ccdb-TTCC-SNAC-6xhis
247405cwby0055GGA cloning AGGA-HALC2_068-GS-promoter-ccdb-TTCC-SNAC-6xhis
247406cwby0056GGA cloning AGGA-HALC3_104-GS-promoter-ccdb-TTCC-SNAC-6xhis
247407cwby0057GGA cloning AGGA-HALC3_109-GS-promoter-ccdb-TTCC-SNAC-6xhis
247408cwby0058GGA cloning AGGA-HALC3_110-GS-promoter-ccdb-TTCC-SNAC-6xhis
247409cwby0059GGA cloning AGGA-HALC3_114-GS-promoter-ccdb-TTCC-SNAC-6xhis
247410cwby0060GGA cloning AGGA-HALC3_118-GS-promoter-ccdb-TTCC-SNAC-6xhis
247411cwby0061GGA cloning AGGA-HALC3_919-GS-promoter-ccdb-TTCC-SNAC-6xhis
247412cwby0062GGA cloning AGGA-SB175-GS-promoter-ccdb-TTCC-SNAC-6xhis
247415cwby0065GGA cloning AGGA-HALC4_140-GS-promoter-ccdb-TTCC-SNAC-6xhis
247416cwby0066GGA cloning AGGA-HALC5_169-GS-promoter-ccdb-TTCC-SNAC-6xhis
247417cwby0067GGA cloning AGGA-HALC5_176-GS-promoter-ccdb-TTCC-SNAC-6xhis
247419cwby0069GGA cloning AGGA-promoter-ccdb-TTCC-GS-C4-71-8-SNAC-6xhis
247420cwby0070GGA cloning AGGA-promoter-ccdb-TTCC-GS-C4-81-4-SNAC-6xhis
247422cwby0072GGA cloning AGGA-promoter-ccdb-TTCC-GS-C6-79-10-SNAC-6xhis
247423cwby0073GGA cloning AGGA-promoter-ccdb-TTCC-GS-C8-71-7-1-SNAC-6xhis
247426cwby0076GGA cloning AGGA-C2-02802-GS-promoter-ccdb-TTCC-SNAC-6xhis
247427cwby0077GGA cloning AGGA-C4-71-8-GS-promoter-ccdb-TTCC-SNAC-6xhis
247428cwby0078GGA cloning AGGA-C4-81-4-GS-promoter-ccdb-TTCC-SNAC-6xhis
247429cwby0079GGA cloning AGGA-C6-71-9-1-GS-promoter-ccdb-TTCC-SNAC-6xhis
247432cwby0082GGA cloning AGGA-H6-GS-promoter-ccdb-TTCC-SNAC-6xhis
247433cwby0083GGA cloning AGGA-H8-GS-promoter-ccdb-TTCC-SNAC-6xhis
247435DMX0002Demultiplexing M-BsaI_AGGA_BsmBI-promoter-ccdb-BsmBI_TTCC_BsaI_W-his__AMPR

Antibodies from Article