Split-Small GTPase Reassembly as a Method to Control Cellular Signaling with User-Defined Inputs.
He Y, Faulkner BM, Weatherford RS, Hyun E, Stains CI
ACS Chem Biol. 2025 Aug 26. doi: 10.1021/acschembio.5c00083.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232262 | pIRES-mCerulean-GAI1-92-KRas/N12-KRas/13C(Q61L)-GID1-mVenus-CAAX | Express split-KRas fragments that are fused with CIDs and FPs |
| 232263 | pIRES-mCerulean-GAI1-92-KRas/N12-KRas/13C(S17N)-GID1-mVenus-CAAX | Express split-KRas fragments that are fused with CIDs and FPs |
| 232264 | pIRES-mCerulean-GID1-Cdc42/N12-Cdc42/13C(Q61L)-GAI1-92-mVenus-CAAX | Express split-Cdc42 fragments that are fused with CIDs and FPs |
| 232265 | pIRES-mCerulean-GID1-Cdc42/N12-Cdc42/13C(T17N)-GAI1-92-mVenus-CAAX | Express split-Cdc42 fragments that are fused with CIDs and FPs |
| 232266 | pIRES-mCerulean-ABI-RhoA/N12-RhoA/13C(Q63L)-PYL-mVenus-CAAX | Express split-RhoA fragments that are fused with CIDs and FPs |
| 232267 | pIRES-mCerulean-ABI-RhoA/N12-RhoA/13C(T19N)-PYL-mVenus-CAAX | Express split-RhoA fragments that are fused with CIDs and FPs |