Skip to main content
Addgene

Recognition of BACH1 quaternary structure degrons by two F-box proteins under oxidative stress.

Cao S, Garcia SF, Shi H, James EI, Kito Y, Shi H, Mao H, Kaisari S, Rona G, Deng S, Goldberg HV, Ponce J, Ueberheide B, Lignitto L, Guttman M, Pagano M, Zheng N
Cell. 2024 Oct 30:S0092-8674(24)01188-7. doi: 10.1016/j.cell.2024.10.012. (Link opens in a new window) PubMed (Link opens in a new window) Article


Plasmids from this article have been deposited by multiple labs. See below for all materials from each lab:

  • Michele Pagano
  • Ning Zheng

    • Plasmids from Article

    ID Plasmid Purpose
    228367pFB-GST-FBXO22express human FBXO22 in insect cells, such as Trichoplusia ni Hi5
    228368pFB-His-SKP1express human SKP1 in insect cells, such as Trichoplusia ni Hi5
    228369pACE-MBP-FBXL17 (310–701)express human FBXL17 in insect cells, such as Trichoplusia ni Hi5
    232269pcDNA3.1 HA-BACH1Expression of N-terminal tagged HA-BACH1 (H. sapiens) in human cell lines.
    232271pcDNA3.1 HA-FBXO22Expression of N-terminal tagged HA-FBXO22 (H. sapiens) in human cell lines
    232273pcDNA3.1 FFSS-BACH1Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) in human cell lines
    232274pcDNA3.1 FFSS-BACH1 C122EExpression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) with Cys122 to Glu point mutation in human cell lines
    232275pcDNA3.1 FLAG-BACH2Expression of FLAG-tagged BACH2 (H. sapiens) in human cell lines
    236188FLAG-FBXL17Expression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.

    Antibodies from Article