A single-chain antibody-based AID2 system for conditional degradation of GFP-tagged and untagged proteins.
Islam M, Negishi T, Kitamoto N, Hatoyama Y, Gamo K, Hayashi KI, Kanemaki MT
J Cell Sci. 2025 Jun 9:jcs.263961. doi: 10.1242/jcs.263961.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232297 | mAID(WT)–GFP NB(WT) | WT GFP nanbody adaptor |
| 232298 | mAID(KR)–GFP NB(KR) | KR GFP nanobody adaptor |
| 232299 | mAID(KR)–GFP NB(WT) | Hybrid GFP adaptor |
| 232300 | mAID(KR)–p53 NB(KR) | KR p53 adaptor |
| 232301 | mAID(KR)–p53 NB(WT) | Hybrid p53 adaptor |
| 232302 | mAID(KR)–RAS MB(KR) | KR RAS adaptor |
| 232303 | mAID(KR)–RAS MB(WT) | Hybrid RAS adaptor |
| 232304 | mAID(KR)–2×GFP NB(WT) | KR GFP adaptor for C. elegans |