Quanfu Ma Lab: Mechanism of Isoleucyl-tRNA synthetase 2 regulating proliferation and apoptosis of cervical cancer cells

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
232336	pcDNA3.1-eIF4E	Expresses human eIF4E (ENST00000450253.7, NM_001968.5) in mammalian cells
232337	pcDNA3.1-IARS2	Expresses human IARS2 (ENST00000366922.3, NM_018060.4) in mammalian cells
232338	pcDNA3.1-IARS2-H112A/P115A	Expresses human IARS2 with putative isoleucine binding deficiency mutations (H112A and P115A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340
232339	pcDNA3.1-IARS2-K664A/K667A	Expresses human IARS2 with putative tRNA binding deficiency mutations (K664A and K667A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340
232340	pcDNA3.1-IARS2-tb	Expresses human IARS2 with synonymous mutations (to prevent recognition by siRNA) in mammalian cells
232341	pcDNA3.1-flag-IARS2-Δ1-48	Expresses human IARS2 with mito-location signal replaced by flag tag in mammalian cells, also carrying synonymous mutations described in Addgene #232340