ATLAS: a rationally designed anterograde transsynaptic tracer.
Rivera JF, Huang H, Weng W, Sohn H, Girasole AE, Li S, Albanese MA, Qin M, Tao C, Klug ME, Rao S, Paletzki R, Herring BE, Kanoski SE, Zhang LI, Gerfen CR, Sabatini BL, Arnold DB
Nat Methods. 2025 May;22(5):1101-1111. doi: 10.1038/s41592-025-02670-x. Epub 2025 May 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232351 | pAAV-ATLASsnCre | Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells. |
| 232352 | pAAV-DIO-ATLASsnFlp | Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells. |
| 232353 | pAAV-DIO-BACE-HA | Beta-Secretase to be expressed (1:1) with either ATLAS protein. |