Engineering inducible signaling receptors to enable erythropoietin-free erythropoiesis
Shah AP, Majeti KR, Ekman FK, Selvaraj S, Soupene E, Chati P, Sinha R, Luna SE, Charlesworth CT, McCreary T, Lesch BJ, Tran T, Sharma D, Chu SN, Porteus MH, Cromer MK
bioRxiv 2024.04.12.589260
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232412 | MKC131_CCR5(SFFV-synEPOR-2A-YFP) | AAV production plasmid for SFFV(synEPOR) vector from Figs. 2-3 that mediates HDR at CCR5 locus using CCR5 gRNA. YFP is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs. |
| 232413 | MKC175_HBA1reg(synEPOR) | AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank H+C15BA1 cassette. HAs are ~400bp each. |
| 232414 | MKC176_EPOR(2A-synEPOR) | AAV production plasmid for EPOR(synEPOR) vector from Figs. 3-5 that mediates HDR at EPOR locus using EPOR gRNA. synEPOR is codon diverged to prevent premature recombination with EPOR locus. |
| 232415 | MKC177_CCR5(PGK-synEPOR) | AAV production plasmid for PGK(synEPOR) vector from Figs. 3-5 that mediates HDR at CCR5 locus using CCR5 gRNA. synEPOR is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs. |