Protein structure and interactions elucidated with in-cell NMR for different cell cycle phases and in 3D human tissue models.
Rynes J, Istvankova E, Dzurov Krafcikova M, Luchinat E, Barbieri L, Banci L, Kamarytova K, Loja T, Fafilek B, Rico-Llanos G, Krejci P, Macurek L, Foldynova-Trantirkova S, Trantirek L
Commun Biol. 2025 Feb 7;8(1):194. doi: 10.1038/s42003-025-07607-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232477 | pB-CuR-hSOD1 | The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end |
| 232478 | pB-TR-hSOD1 | Tetracycline inducible PiggyBac vector expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end |
| 232479 | pB-TR-TTHA | Tetracycline inducible PiggyBac vector expressing bacterial TTHA1718 (TTHA) gene gene including flag-tag (DYKDDDDK) on the 3' end |
| 232480 | pB-TR-hCAII | Tetracycline inducible PiggyBac vector expressing human CAII gene including flag-tag (DYKDDDDK) on the 3' end |
| 232481 | pHLsec hSOD1 | vector for transient transfection expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end |