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Plasmid sequences and availability of a two-plasmid system for CRISPRi knockdown of Clostridioides difficile genes without antibiotic selection.

Chua M, Erickson D, Collins J
Microbiol Resour Announc. 2025 Apr 28:e0011625. doi: 10.1128/mra.00116-25. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
232482pJAK184.tetR.PT5-3.dCas9optimized ahTet inducible dCas9 for insertion into C. difficile genome
232483pJC.15A.sgRNA.TAsgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.

Antibodies from Article