Plasmid sequences and availability of a two-plasmid system for CRISPRi knockdown of Clostridioides difficile genes without antibiotic selection
Collins J
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232482 | pJAK184.tetR.PT5-3.dCas9 | optimized ahTet inducible dCas9 for insertion into C. difficile genome |
| 232483 | pJC.15A.sgRNA.TA | sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection. |