Plasmid sequences and availability of a two-plasmid system for CRISPRi knockdown of Clostridioides difficile genes without antibiotic selection.

Chua M, Erickson D, Collins J
Microbiol Resour Announc. 2025 Apr 28:e0011625. doi: 10.1128/mra.00116-25. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
232482	pJAK184.tetR.PT5-3.dCas9	optimized ahTet inducible dCas9 for insertion into C. difficile genome
232483	pJC.15A.sgRNA.TA	sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.