Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a Host for Lactose Valorization.
Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, Martin VJJ
ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233473 | pMTKM01 | SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and Nat selection. |
| 233474 | pMTKM02 | SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-Nat selection. |
| 233475 | pMTKM03 | SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and G418 selection. |
| 233476 | pMTKM04 | SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-G418 selection. |
| 233477 | pMTKM05 | SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and Hyg selection. |
| 233478 | pMTKM06 | SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-Hyg selection. |
| 233479 | pMTKM07 | SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and URA selection. |
| 233480 | pMTKM08 | SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-URA selection. |
| 233481 | pMTKM09 | Level 0 plasmid for MoClo, encodes the PST1 promoter for K.marxianus |
| 233482 | pMTKM10 | Level 0 plasmid for MoClo, encodes the GLK1A promoter for K.marxianus |
| 233483 | pMTKM11 | Level 0 plasmid for MoClo, encodes the GLK1B promoter for K.marxianus |
| 233484 | pMTKM12 | Level 0 plasmid for MoClo, encodes the SCL1 promoter for K.marxianus |
| 233485 | pMTKM13 | Level 0 plasmid for MoClo, encodes the ZWF promoter for K.marxianus |
| 233486 | pMTKM14 | Level 0 plasmid for MoClo, encodes the RPE1 promoter for K.marxianus |
| 233487 | pMTKM15 | Level 0 plasmid for MoClo, encodes the GPD1 promoter for K.marxianus |
| 233488 | pMTKM16 | Level 0 plasmid for MoClo, encodes the ALD2 promoter for K.marxianus |
| 233489 | pMTKM17 | Level 0 plasmid for MoClo, encodes the HSP26 promoter for K.marxianus |
| 233490 | pMTKM18 | Level 0 plasmid for MoClo, encodes the HSP60 promoter for K.marxianus |
| 233491 | pMTKM19 | Level 0 plasmid for MoClo, encodes the PIR1 promoter for K.marxianus |
| 233492 | pMTKM20 | Level 0 plasmid for MoClo, encodes the SSA3 promoter for K.marxianus |
| 233493 | pMTKM21 | Level 0 plasmid for MoClo, encodes the SOD1 promoter for K.marxianus |
| 233494 | pMTKM22 | Level 0 plasmid for MoClo, encodes the INU1 promoter for K.marxianus |
| 233495 | pMTKM23 | Level 0 plasmid for MoClo, encodes the ADH1 promoter for K.marxianus |
| 233496 | pMTKM24 | Level 0 plasmid for MoClo, encodes the PGK promoter for K.marxianus |
| 233497 | pMTKM25 | Level 0 plasmid for MoClo, encodes the TEF3 promoter for K.marxianus |
| 233498 | pMTKM26 | Level 0 plasmid for MoClo, encodes the TDH3 promoter for K.marxianus |
| 233499 | pMTKM27 | Level 0 plasmid for MoClo, encodes the HFF1 promoter for K.marxianus |
| 233500 | pMTKM28 | Level 0 plasmid for MoClo, encodes the NC1 promoter for K.marxianus |
| 233501 | pMTKM29 | Level 0 plasmid for MoClo, encodes the COX20 promoter for K.marxianus |
| 233502 | pMTKM30 | Level 0 plasmid for MoClo, encodes inducible TDH3-tetO2p for K. marxianus |
| 233503 | pMTKM31 | Level 0 plasmid for MoClo, encodes inducible TEF3-tetO2p for K. marxianus |
| 233504 | pMTKM32 | Level 0 plasmid for MoClo, encodes inducible PGK1-tetO2p for K. marxianus |