Compact transcription factor cassettes generate functional, engraftable motor neurons by direct conversion.
Wang NB, Adewumi HO, Lende-Dorn BA, Beitz AM, O'Shea TM, Galloway KE
Cell Syst. 2025 Mar 10, Volume 16, Issue 4 :101206. doi: 10.1016/j.cels.2025.101206
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PubMed
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233185 | pMXs-HRAS(G12V)-IRES-p53DD | Retroviral expression of HRAS(G12V) and p53DD (RIDD) |
| 233186 | pENTR-HRAS(G12V)-IRES-p53DD | Gateway entry vector for HRAS(G12V) and p53DD (RIDD) |
| 233187 | pMXs-p53DD-IRES-HRAS(G12V) | Retroviral expression of p53DD and HRAS(G12V) (DDIR) |
| 233188 | pENTR-p53DD-IRES-HRAS(G12V) | Gateway entry vector for p53DD and HRAS(G12V) (DDIR) |
| 233189 | pMXs-mNgn2-x3HA | Retroviral expression of tagged mNgn2x3HA |
| 233190 | pMXs-mNgn2-x3FLAG | Retroviral expression of tagged mNgn2x3FLAG |
| 233191 | pMXs-mNgn2-x1HA | Retroviral expression of tagged mNgn2x1HA |
| 233192 | pMXs-NIL (with mNgn2x3HA) | Retroviral expression of Ngn2x3HA, Isl1, and Lhx3 for motor neuron reprogramming |
| 233193 | pMXs-NLI (with mNgn2x3HA) | Retroviral expression of Ngn2x3HA, Lhx3, and Isl1 for motor neuron reprogramming |
| 233194 | pMXs-LIN (with mNgn2x3HA) | Retroviral expression of Lhx3, Isl1, and Ngn2x3HA for motor neuron reprogramming |
| 233195 | pMXs-LNI (with mNgn2x3HA) | Retroviral expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming |
| 233196 | pMXs-ILN (with mNgn2x3HA) | Retroviral expression of Isl1, Lhx3, and Ngn2x3HA for motor neuron reprogramming |
| 233197 | pMXs-INL (with mNgn2x3HA) | Retroviral expression of Isl1, Ngn2x3HA, and Lhx3 for motor neuron reprogramming |
| 233198 | pMXs-SNAP-p53DD-IRES-HRAS(G12V) | Retroviral expression of SNAP-p53DD and HRAS(G12V) |
| 233199 | pENTR-HRAS(G12V)-IRES-SNAP-p53DD | Gateway entry vector for HRAS(G12V) and SNAP-p53DD |
| 233200 | pMXs-HRAS(G12V)-IRES-SNAP-p53DD | Retroviral expression of HRAS(G12V) and SNAP-p53DD |
| 233201 | pENTR-SNAP-p53DD-IRES-HRAS(G12V) | Gateway entry vector for SNAP-p53DD and HRAS(G12V) |
| 233202 | pMXs-rtTA | Retroviral expression of rtTA for dox-inducible activation from a TRE promoter |
| 233203 | pAAV-CMV-LNI-bGH | AAV expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming |
| 233204 | pAAV-CAG-SNAP-p53DD-IRES-HRAS(G12V)-bGH | AAV expression of SNAP-p53DD and HRAS(G12V) |
| 233205 | pFUW-LNI | Lentiviral dox-inducible expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming |
| 233206 | pFUW-SNAP-p53DD-IRES-HRAS(G12V) | Lentiviral dox-inducible expression of SNAP-p53DD and HRAS(G12V) |
| 233207 | pMXs-jRCaMP1b | Retroviral expression of jRCaMP1b calcium reporter |