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Multiplexing light-inducible recombinases to control cell fate, Boolean logic, and cell patterning in mammalian cells.

Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. Epub 2025 May 9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
233652IK216Cre BFP Reporter
233653IK331Dox-inducible blue light-inducible Cre-N
233654IK332Dox-inducible blue light-inducible Cre-C
233655IK349Dox-inducible red light-inducible Flp-C
233656IK350Dox-inducible red light-inducible Flp-N
233657CT751Dox-inducible VP64-MyoD
233658CT752Flp GFP Reporter
233659CT754FLEx switch with tTA
233660CT691BLADE
233661CT769Split Flp on same plasmid
233662CT336AND Gate Reporter
233663CT447Red light-inducible Flp-C
233664CT443Red light-inducible Flp-C
233665CT452Red light-inducible Flp-N
233666CT438Red light-inducible Flp-N
233667CT450Red light-inducible Flp-N
233668CT445Red light-inducible Flp-C
233669CT611Red light-inducible Cre-C
233670CT612Red light-inducible Cre-C
233671CT614Red light-inducible Cre-C
233672CT361Red light-inducible Cre-C
233673CT367Red light-inducible Cre-N
233674CT363Red light-inducible Cre-C
233675CT616Red light-inducible Cre-N
233676CT617Red light-inducible Cre-N
233677CT619Red light-inducible Cre-N
233678CT366Red light-inducible Cre-N
233679CT362Red light-inducible Cre-C
233680CT368Red light-inducible Cre-N
233681CT533Red light-inducible Vcre-N
233682CT534Red light-inducible Vcre-C

Antibodies from Article