Multiplexing light-inducible recombinases to control cell fate, Boolean logic, and cell patterning in mammalian cells.
Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. Epub 2025 May 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233652 | IK216 | Cre BFP Reporter; loxP-STOP-loxP-BFP |
| 233653 | IK331 | Dox-inducible blue light-inducible Cre-N; Cre-N229-pmag |
| 233654 | IK332 | Dox-inducible blue light-inducible Cre-C; Cre-230C-nmag |
| 233655 | IK349 | Dox-inducible red light-inducible Flp-C; Flp-397C-PhyA |
| 233656 | IK350 | Dox-inducible red light-inducible Flp-N; Flp-N396-FHY1 |
| 233657 | CT751 | Dox-inducible VP64-MyoD |
| 233658 | CT752 | Flp GFP Reporter; FRT-STOP-FRT-GFP |
| 233659 | CT754 | FLEx switch with tTA |
| 233660 | CT691 | BLADE (two-input, four-output decoder); Cre excises lox sites to express GFP, Flp excises FRT sites to express iRFP, and the presence of both recombinases is designed to express mRuby. |
| 233661 | CT769 | Split Flp on same plasmid; PhyA-Flp-397-Flp-396-FHY1 |
| 233662 | CT336 | AND Gate Reporter to identify Cre and Flp recombination; FRT-STOP-FRT-loxP-STOP-loxP-GFP |
| 233663 | CT447 | Red light-inducible Flp-C; Flp-28C-FHY1 |
| 233664 | CT443 | Red light-inducible Flp-C; Flp-375C-PhyA |
| 233665 | CT452 | Red light-inducible Flp-N; Flp-N396-FHY1 |
| 233666 | CT438 | Red light-inducible Flp-N; Flp-N27-PhyA |
| 233667 | CT450 | Red light-inducible Flp-N; Flp-N374-FHY1 |
| 233668 | CT445 | Red light-inducible Flp-C; Flp-397C-PhyA |
| 233669 | CT611 | Red light-inducible Cre-C; Cre-230C-FHY1 |
| 233670 | CT612 | Red light-inducible Cre-C; Cre-252C-FHY1 |
| 233671 | CT614 | Red light-inducible Cre-C; Cre-271C-FHY1 |
| 233672 | CT361 | Red light-inducible Cre-C; Cre-106C-PhyA |
| 233673 | CT367 | Red light-inducible Cre-N; Cre-N229-FHY1 |
| 233674 | CT363 | Red light-inducible Cre-C; Cre-252C-PhyA |
| 233675 | CT616 | Red light-inducible Cre-N; Cre-N229-PhyA |
| 233676 | CT617 | Red light-inducible Cre-N; Cre-N251-PhyA |
| 233677 | CT619 | Red light-inducible Cre-N; Cre-N270-PhyA |
| 233678 | CT366 | Red light-inducible Cre-N; Cre-N105-FHY1 |
| 233679 | CT362 | Red light-inducible Cre-C; Cre-230C-PhyA |
| 233680 | CT368 | Red light-inducible Cre-N; Cre-N251-PhyA |
| 233681 | CT533 | Red light-inducible Vcre-N; Vcre-N277-FHY1 |
| 233682 | CT534 | Red light-inducible Vcre-C; Vcre-278C-PhyA |