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Identification of polyphosphate-binding proteins in E. coli uncovers targets involved in translation control and ribosome biogenesis

Baijal K, Kore B, Abramchuk I, Denoncourt A, Han S, Simms A, Dagenais A, Long AR, Rudner AD, Lavallée-Adam M, Gray MJ, Downey M
bioRxiv 2025.02.12.637445 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
232578pRnr-CSDExpression of the Rnr cold shock domains I and II (residues 1 to 648) as a GST-fusion.
232579pRnr-NDExpression of the Rnr nuclease domain (residues 649 to 1929) as a GST-fusion.
232580pRnr-S1BDExpression of the Rnr S1 and basic domains (residues 1930 to 2442) as a GST-fusion.
233088pYihIExpression of GST-YihI (wildtype sequence)
233091pYihI-N-term K-RExpression of GST-YihI with N-term lysines (K) mutated to arginine (R)
233092pYihI-C-term K-RExpression of GST-YihI with C-term lysines (K) mutated to arginine (R)
233093pYihI-N+C-term K-RExpression of GST-YihI with N+C-term lysines (K) mutated to arginine (R)
233094pYihI-All K-RExpression of GST-YihI with all lysines (K) mutated to arginine (R)
233095pYihI-N-term D-N/E-QExpression of GST-YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
233096pYihI-N-term S-AExpression of GST-YihI with N-term serines (S) mutated to alanine (A)
233097pYihI-N-term D-A/E-LExpression of GST-YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
233713pScPPX1Bacterial expression of the yeast PPX1 gene

Antibodies from Article