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Identification of polyphosphate-binding proteins in E. coli uncovers targets involved in translation control and ribosome biogenesis

Baijal K, Kore B, Abramchuk I, Denoncourt A, Han S, Simms A, Dagenais A, Long AR, Rudner AD, Lavallée-Adam M, Gray MJ, Downey M
bioRxiv 2025.02.12.637445 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
233088pYihIExpression of GST-YihI (wildtype sequence)
233095pYihI-N-term D-N/E-QExpression of GST-YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
233096pYihI-N-term S-AExpression of GST-YihI with N-term serines (S) mutated to alanine (A)
233097pYihI-N-term D-A/E-LExpression of GST-YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
233713pScPPX1Bacterial expression of the yeast PPX1 gene

Antibodies from Article