Identification of polyphosphate-binding proteins in Escherichia coli uncovers targets involved in translation control and ribosome biogenesis.
Baijal K, Kore B, Abramchuk I, Denoncourt A, Han S, Simms A, Dagenais A, Long AR, Rudner AD, Lavallee-Adam M, Gray MJ, Downey M
mBio. 2025 Aug 13;16(8):e0050025. doi: 10.1128/mbio.00500-25. Epub 2025 Jul 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232578 | pRnr-CSD | Expression of the Rnr cold shock domains I and II (residues 1 to 648) as a GST-fusion. |
| 232579 | pRnr-ND | Expression of the Rnr nuclease domain (residues 649 to 1929) as a GST-fusion. |
| 232580 | pRnr-S1BD | Expression of the Rnr S1 and basic domains (residues 1930 to 2442) as a GST-fusion. |
| 233088 | pYihI | Expression of GST-YihI (wildtype sequence) |
| 233091 | pYihI-N-term K-R | Expression of GST-YihI with N-term lysines (K) mutated to arginine (R) |
| 233092 | pYihI-C-term K-R | Expression of GST-YihI with C-term lysines (K) mutated to arginine (R) |
| 233093 | pYihI-N+C-term K-R | Expression of GST-YihI with N+C-term lysines (K) mutated to arginine (R) |
| 233094 | pYihI-All K-R | Expression of GST-YihI with all lysines (K) mutated to arginine (R) |
| 233095 | pYihI-N-term D-N/E-Q | Expression of GST-YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q) |
| 233096 | pYihI-N-term S-A | Expression of GST-YihI with N-term serines (S) mutated to alanine (A) |
| 233097 | pYihI-N-term D-A/E-L | Expression of GST-YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L) |
| 233713 | pScPPX1 | Bacterial expression of the yeast PPX1 gene |