A Standardized Set of MoClo-Compatible Inducible Promoter Systems for Tunable Gene Expression in Yeast.
O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N, Hasty J
ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233805 | pRO129 | MoClo-YTK; type3; rtTA systems; rtTA3 |
| 233806 | pRO112 | MoClo-YTK; type3; rtTA systems; rtTA-v10 |
| 233807 | pRO217 | MoClo-YTK; type3; rtTA systems; rtTA-v16 |
| 233808 | pRO232 | MoClo-YTK; type2A; ABA systems; phlfO7 |
| 233809 | pRO174 | MoClo-YTK; type3A; ABA systems; Phlf-NES |
| 233810 | pRO175 | MoClo-YTK; type3B; ABA systems; ABI |
| 233811 | pRO177 | MoClo-YTK; type3B; ABA systems; PYL1 |
| 233812 | pRO286 | MoClo-YTK; type2A; Auxin systems; amtRO7 |
| 233813 | pRO271 | MoClo-YTK; type3A; Auxin systems; AmtR-NES |
| 233814 | pWN022 | MoClo-YTK; type3B; Auxin systems; AIDΔ34 |
| 233815 | pWN050 | MoClo-YTK; type3B; Auxin systems; TIR1-U1 |
| 233816 | pWN083 | MoClo-YTK; type3B; Auxin systems; TIR1-U2 |
| 233817 | pAL009 | MoClo-YTK; type3B; NS3 systems; NS3-V1 |
| 233818 | pWN018 | MoClo-YTK; type3B; NS3 systems; NS3-V2 |
| 233819 | pWN079 | MoClo-YTK; type3B; NS3 systems; NS3a |
| 233820 | pWN080 | MoClo-YTK; type3B; NS3 systems; Linker-NS3a |
| 233821 | pWN043 | MoClo-YTK; type3B; NS3 systems; DNCR2 |
| 233822 | pWN062 | MoClo-YTK; type3A; NS3 systems; LexA-NES |
| 233823 | pRO117 | MoClo-YTK; type3; Reporter; mNeonGreen |
| 233824 | pRO176 | MoClo-YTK; type3A; Activation domains; NLS-VP48 |
| 233825 | pRO275 | MoClo-YTK; type3A; Activation domains; NLS-VP48-1XOaf1 |
| 233826 | pRO276 | MoClo-YTK; type3A; Activation domains; NLS-VP48-2XOaf1 |
| 233827 | pRO288 | MoClo-YTK; type4A; Activation domains; VP48 |
| 233828 | pWN102 | MoClo-YTK; type4A; Activation domains; NLS-VP48-2XOaf1 |
| 233829 | pAL002 | MoClo-YTK; type4A; Activation domains; VP48-2XOaf1 |
| 233830 | pRO211 | MoClo-YTK; ConLS/RE cassette; Const. mNG; pTDH3-mNeonGreen-tENO2 |
| 233831 | pRO261 | MoClo-YTK; ConLS/RE cassette; Const. mNG; pRPL18B-mNeonGreen-tENO2 |
| 233832 | pRO262 | MoClo-YTK; ConLS/RE cassette; Const. mNG; pRNR2-mNeonGreen-tENO2 |
| 233833 | pRO263 | MoClo-YTK; ConLS/RE cassette; Const. mNG; pRAD27-mNeonGreen-tENO2 |
| 233834 | pRO264 | MoClo-YTK; ConLS/RE cassette; Const. mNG; pREV1-mNeonGreen-tENO2 |
| 233835 | pRO227 | MoClo-YTK; ConL1/RE cassette; rtTA systems; tetO7-pLEU2m-mNeonGreen-tENO2 |
| 233836 | pRO229 | MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRPL18B-rtTA-v10-tADH1 |
| 233837 | pRO230 | MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRPL18B-rtTA3-tADH1 |
| 233838 | pRO231 | MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRPL18B-rtTA-v16-tADH1 |
| 233839 | pRO246 | MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRNR2-rtTA-v16-tADH1 |
| 233840 | pRO247 | MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRAD27-rtTA-v16-tADH1 |
| 233841 | pRO253 | MoClo-YTK; ConLS/R1 cassette; rtTA systems; pTDH3-rtTA-v10-tADH1 |
| 233842 | pRO254 | MoClo-YTK; ConLS/R1 cassette; rtTA systems; pTDH3-rtTA3-tADH1 |
| 233843 | pRO204 | MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-ABI-tENO1 |
| 233844 | pRO241 | MoClo-YTK; ConLS/R1 cassette; ABA systems; pTDH3-Phlf-NES-PYL1-tADH1 |
| 233845 | pRO279 | MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-1xOaf1-ABI-tENO1 |
| 233846 | pRO280 | MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1 |
| 233847 | pRO236 | MoClo-YTK; ConL2/RE cassette; ABA systems; phlfO7-pLEU2m-mNeonGreen-tENO2 |
| 233848 | pAL001 | MoClo-YTK; ConL1/RE cassette; NS3 systems; lexAO6-pLEU2m-mNeonGreen-tENO2 |
| 233849 | pAL008 | MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NLS-NS3-V1-VP48-2XOaf1-tADH1 |
| 233850 | pWN020 | MoClo-YTK; ConL2/RE cassette; Auxin systems; amtRO7-pLEU2m-mNeonGreen-tENO2 |
| 233851 | pWN021 | MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NLS-NS3-V2-VP48-2XOaf1-tADH1 |
| 233852 | pWN057 | MoClo-YTK; ConLS/R1 cassette; Auxin systems; pTDH3-AmtR-NES-TIR1-U1-tADH1 |
| 233853 | pWN059 | MoClo-YTK; ConL1/R2 cassette; Auxin systems; pTDH3-NLS-VP48-2XOaf1-AIDΔ34-tENO1 |
| 233854 | pWN061 | MoClo-YTK; ConL1/R2 cassette; NS3 systems; pTDH3-NLS-VP48-2XOaf1-DNCR2-tENO1 |
| 233855 | pWN069 | MoClo-YTK; ConL2/RE cassette; NS3 systems; lexAO6-pLEU2m-mNeonGreen-tENO2 |
| 233856 | pWN075 | MoClo-YTK; ConLS/R1 cassette; Auxin systems; pRPL18B-AmtR-NES-TIR1-U1-tADH1 |
| 233857 | pWN076 | MoClo-YTK; ConLS/R1 cassette; Auxin systems; pRNR2-AmtR-NES-TIR1-U1-tADH1 |
| 233858 | pWN081 | MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3a-VP48-2XOaf1-tADH1 |
| 233859 | pWN086 | MoClo-YTK; ConLS/R1 cassette; Auxin systems; pTDH3-AmtR-NES-TIR1-U2-tADH1 |
| 233860 | pWN103 | MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3-V1-NLS-VP48-2XOaf1-tADH1 |
| 233861 | pWN104 | MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3-V2-NLS-VP48-2XOaf1-tADH1 |
| 233862 | pWN113 | MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-linker-NS3a-tADH1 |
| 233863 | pAL004 | MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2 |
| 233864 | pAL003 | MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRPL18B-rtTA3-tADH1; tetO7-pLEU2m-MCS-tENO2 |
| 233865 | pAL005 | MoClo-YTK URA multigene plasmid with MCS; ABA systems; pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2 |
| 233866 | pAL007 | MoClo-YTK URA multigene plasmid with MCS; NS3 systems; pTDH3-LexA-NLS-NS3-V1-VP48-2xOaf1-tADH1; lexAO6-pLEU2m-MCS-tENO2 |