Cytosolic NADK is conditionally essential for folate-dependent nucleotide synthesis.
Flickinger KM, Mellado Fritz CA, Huggler KS, Wade GM, Chang GR, Fox KC, Simcox JA, Cantor JR
Nat Metab. 2025 May 2. doi: 10.1038/s42255-025-01272-3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233888 | pLJC2-NADK-3xFLAG | lentiviral overexpression vector for NADK |
| 233889 | pLJC2-NADK (D184A)-3xFLAG | lentiviral overexpression vector for NADK (D184A) |
| 233890 | pLJC2-Blast-Rap2A-3xFLAG | lentiviral overexpression vector for RAP2A |
| 233891 | pLJC2-Blast-SHMT2-3xFLAG | lentiviral overexpression vector for SHMT2 |
| 233892 | pLJC2-Blast-DHFR-3xFLAG | lentiviral overexpression vector for DHFR |
| 233893 | pLJC2-Blast-DHFR | lentiviral overexpression vector for DHFR |
| 233894 | pLJC6-NADK (D184A)-3xFLAG | lentiviral overexpression vector NADK (D184A) |
| 233895 | pLJC6-NADK2-3xFLAG | lentiviral overexpression vector for NADK2 |
| 233896 | pLJC6-NADK2(delta MTS)-3xFLAG | lentiviral overexpression vector for NADK2 (delta MTS) |
| 233897 | pLentiCRISPR-v1-sgNADK2 | lentiviral vector expressing Cas9 and an sgRNA targeting NADK2 |
| 233898 | pLentiCRISPR-v1-sgDHFR | lentiviral vector expressing Cas9 and an sgRNA targeting DHFR |