Leveraging CRISPR Cas9 RNPs and Cre-loxP in Picochlorum celeri for generation of field deployable strains and selection marker recycling.
Burch TA, Krishnan A, Posewitz MC
Front Microbiol. 2025 Jul 14;16:1588625. doi: 10.3389/fmicb.2025.1588625. eCollection 2025.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234430 | pNRCreCNAT | Picochlorum celeri CloNat insert with nitrate-inducible Cre recombinase and loxP sites for marker and Cre removal |
| 234431 | pNRCreBle | Picochlorum celeri ShBle insert with nitrate-inducible Cre recombinase and loxP sites for marker and Cre removal |