Near 100% efficient homology-dependent genome engineering in the human fungal pathogen Cryptococcus neoformans

Nalley MJ, Banerjee S, Huang MY, Madhani HD
bioRxiv 2025.02.14.638277 Article

Plasmids from Article

ID	Plasmid	Purpose
234495	pBHM2616	HYGR-CnU6-empty sgRNA
234496	pBHM2617	NATR-CnU6-empty sgRNA
234497	pBHM2618	NEOR-CnU6-empty sgRNA
234498	pBHM2619	amdS-CnU6-empty sgRNA
234500	pBHM2661	Linker-Cno_mNeonGreen-CBP-2xFLAG-CnU6-sgRNA-NEOR
234501	pBHM2662	Linker-Cno_mNeonGreen-CBP-2xFLAG-CnU6-sgRNA-amdS