A general assay platform to study protein pharmacology using ligand-dependent structural dynamics.
Ciulla DA, Dranchak PK, Aitha M, van Neer RHP, Shah D, Tharakan R, Wilson KM, Wang Y, Braisted JC, Inglese J
Nat Commun. 2025 May 10;16(1):4342. doi: 10.1038/s41467-025-59658-6.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207117 | FLuc-N-HiBiT | Bacterially expressed HiBiT-FLuc for Ni-NTA purification |
| 207118 | FLuc-C-HiBiT | Bacterially expressed FLuc-HiBiT for Ni-NTA purification |
| 207119 | DHFR-C-HiBiT | Bacterially expressed DHFR-HiBiT for Ni-NTA purification |
| 207120 | Ce iPGM-C-HiBiT | Bacterially expressed iPGM-HiBiT for Ni-NTA purification |
| 207121 | ABL1-N-HiBiT kinase domain_v1 | Bacterially expressed HiBiT-ABL1 kinase domain (D233-S500) for Ni-NTA purification |
| 211377 | PKA-N-HiBiT | Bacterially expressed HiBiT-PKA for Ni-NTA purification |
| 215437 | E. coli Lig-N-HiBiT | Bacterially expressed E. coli Lig-N-HiBiT for Ni-NTA purification |
| 215438 | E. coli Lig-C-HiBiT | Bacterially expressed E. coli Lig-C-HiBiT for Ni-NTA purification |
| 215441 | T7 Lig-C-HiBiT | Bacterially expressed T7 Lig-C-HiBit for Ni-NTA purification |
| 215479 | T7 Lig-N-HiBiT | Bacterially expressed T7 Lig-N-HiBiT for Ni-NTA purification |
| 218483 | Bm iPGM-HiBiT | Bacterially expressed iPGM-HiBiT for Ni-NTA purification |
| 234566 | FLuc-C-NLuc | Bacterially expressed FLuc-C-NLuc for Ni-NTA purification |
| 234567 | NLuc-His10 | Bacterially expressed NLuc for Ni-NTA purification |
| 234568 | ABL1-C-HiBiT | Bacterially expressed ABL1-C-HiBiT for Ni-NTA purification |