Modular cloning of multigene vectors for the baculovirus system and yeast.
Lai Z, Flanigan SF, Boudes M, Davidovich C
J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
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PubMed
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Article
A subset of these plasmids is included in the MoClo Baculo Toolkit.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 228667 | pMB.001 | Moclo Baculo toolkit level 1 backbone |
| 228668 | pMB.002 | Moclo Baculo toolkit level 2 backbone |
| 228669 | pMB.003 | Recombination to BV DNA (Tn7) |
| 228670 | pMB.004 | Recombination to BV DNA + SpecR (Tn7, SpecR) |
| 228671 | pMB.005 | polH Promoter for Baculovirus expression without Nterminal tag |
| 228672 | pMB.006 | polH Promoter for Baculovirus expression with Nterminal tag |
| 228673 | pMB.007 | tSV40 Terminator for Baculovirus expression without Cterminal tag |
| 228674 | pMB.008 | tSV40 Terminator for Baculovirus expression with Cterminal tag |
| 228675 | pMB.BIG1a | Moclo Baculo toolkit level 2 backbone (biGBac compatible) |
| 228676 | pMB.BIG1b | Moclo Baculo toolkit level 2 backbone (biGBac compatible) |
| 228677 | pMB.BIG1c | Moclo Baculo toolkit level 2 backbone (biGBac compatible) |
| 228678 | pMB.BIG1d | Moclo Baculo toolkit level 2 backbone (biGBac compatible) |
| 228679 | pMB.BIG1e | Moclo Baculo toolkit level 2 backbone (biGBac compatible) |
| 228680 | pMY.001 | pTDH3 Promoter for yeast expression with Nterminal tag |
| 228681 | pMY.002 | pCCW12 Promoter for yeast expression with Nterminal tag |
| 228682 | pMY.003 | pPGK1 Promoter for yeast expression with Nterminal tag |
| 228683 | pMY.004 | pHHF2 Promoter for yeast expression with Nterminal tag |
| 228684 | pMY.005 | pTEF1 Promoter for yeast expression with Nterminal tag |
| 228685 | pMY.006 | pTEF2 Promoter for yeast expression with Nterminal tag |
| 228686 | pMY.007 | pGAL1 Promoter for yeast expression with Nterminal tag |
| 228687 | pM.001 | N terminal MBP affinity tag with PreScission Protease cleavage site |
| 228688 | pM.002 | C terminal MBP affinity tag with PreScission Protease cleavage site |
| 228689 | pM.003 | N terminal dual protein A affinity tag with TEV cleavage site |
| 228690 | pM.004 | C terminal dual protein A affinity tag with TEV cleavage site |
| 228691 | pM.005 | N terminal mTurquoise2 fluorescence tag |
| 228692 | pM.006 | N terminal Venus fluorescence tag |
| 228693 | pM.007 | N terminal mRuby2 fluorescence tag |
| 228694 | pM.008 | N terminal 3xFlag-6xHis affinity tag |
| 228695 | pM.009 | N terminal Strep affinity tag with TEV cleavage site |
| 228696 | pM.010 | C terminal Strep affinity tag with TEV cleavage site |
| 234569 | pCDS.EED | Coding sequence for EED |
| 234570 | pCDS.EZH2 | Coding sequence for EZH2 |
| 234571 | pCDS.RBBP4 | Coding sequence for RBBP4 |
| 234572 | pCDS.SUZ12 | Coding sequence for SUZ12 |
| 234573 | pCDS.AEBP2.I3 | Coding sequence for AEBP2 Isoform 3 |
| 234574 | pMB.M.E2 | Expression of MBP-EZH2 |
| 234575 | pMB.M.S12 | Expression of MBP-SUZ12 |
| 234576 | pMB.M.ED | Expression of MBP-EED |
| 234577 | pMB.M.R4 | Expression of MBP-RBBP4 |
| 234578 | pMB.AI3 | Expression of AEBP2 [Isoform 3] |
| 234579 | pMB.PRC2 | Expression of MBP-EZH2, MBP-SUZ12, MBP-EED and MBP-RBBP4 |
| 234580 | pMB.PRC2.AEBP2 | Expression of MBP-EZH2, MBP-SUZ12, MBP-EED, MBP-RBBP4 and AEBP2 [Isoform 3] |
| 234581 | pMB.BIG1a.PRC2 | Expression of EZH2, MBP-SUZ12, EED, RBBP4 |
| 234582 | pMY.PRC2 | Expression of ProtA-EZH2, SUZ12, EED and RBBP4 |
| 234583 | pMB.Pos1 | Moclo Baculo toolkit level 1 backbone with ConLS/ConR1 and Tn7L |
| 234584 | pMB.Pos2 | Moclo Baculo toolkit level 1 backbone with ConL1/ConR2 and Tn7L |
| 234585 | pMB.Pos3 | Moclo Baculo toolkit level 1 backbone with ConL2/ConR3 and Tn7L |
| 234586 | pMB.Pos4 | Moclo Baculo toolkit level 1 backbone with ConL3/ConR4 and Tn7L |
| 234587 | pMB.Pos5 | Moclo Baculo toolkit level 1 backbone with ConL4/ConR5 and Tn7L |
| 234588 | pMB.Pos6 | Moclo Baculo toolkit level 1 backbone with ConL5/ConRE and Tn7L |
| 234589 | pM.L3456 | Linker plasmid (connect pMB.Pos2 to pMB.002) |
| 234590 | pM.L456 | Linker plasmid (connect pMB.Pos3 to pMB.002) |
| 234591 | pM.L56 | Linker plasmid (connect pMB.Pos4 to pMB.002) |
| 234592 | pM.L6 | Linker plasmid (connect pMB.Pos5 to pMB.002) |
| 234593 | pM.000 | pYTK001 with RFP dropout |