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Modular cloning of multigene vectors for the baculovirus system and yeast.

Lai Z, Flanigan SF, Boudes M, Davidovich C
J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
234569pCDS.EEDCoding sequence for EED
234570pCDS.EZH2Coding sequence for EZH2
234571pCDS.RBBP4Coding sequence for RBBP4
234572pCDS.SUZ12Coding sequence for SUZ12
234573pCDS.AEBP2.I3Coding sequence for AEBP2 Isoform 3
234574pMB.M.E2Expression of MBP-EZH2
234575pMB.M.S12Expression of MBP-SUZ12
234576pMB.M.EDExpression of MBP-EED
234577pMB.M.R4Expression of MBP-RBBP4
234578pMB.AI3Expression of AEBP2 [Isoform 3]
234579pMB.PRC2Expression of MBP-EZH2, MBP-SUZ12, MBP-EED and MBP-RBBP4
234580pMB.PRC2.AEBP2Expression of MBP-EZH2, MBP-SUZ12, MBP-EED, MBP-RBBP4 and AEBP2 [Isoform 3]
234581pMB.BIG1a.PRC2Expression of EZH2, MBP-SUZ12, EED, RBBP4
234582pMY.PRC2Expression of ProtA-EZH2, SUZ12, EED and RBBP4
234583pMB.Pos1Moclo Baculo toolkit level 1 backbone with ConLS/ConR1 and Tn7L
234584pMB.Pos2Moclo Baculo toolkit level 1 backbone with ConL1/ConR2 and Tn7L
234585pMB.Pos3Moclo Baculo toolkit level 1 backbone with ConL2/ConR3 and Tn7L
234586pMB.Pos4Moclo Baculo toolkit level 1 backbone with ConL3/ConR4 and Tn7L
234587pMB.Pos5Moclo Baculo toolkit level 1 backbone with ConL4/ConR5 and Tn7L
234588pMB.Pos6Moclo Baculo toolkit level 1 backbone with ConL5/ConRE and Tn7L
234589pM.L3456Linker plasmid (connect pMB.Pos2 to pMB.002)
234590pM.L456Linker plasmid (connect pMB.Pos3 to pMB.002)
234591pM.L56Linker plasmid (connect pMB.Pos4 to pMB.002)
234592pM.L6Linker plasmid (connect pMB.Pos5 to pMB.002)
234593pM.000pYTK001 with RFP dropout

Antibodies from Article