Metastable condensates suppress conversion to amyloid fibrils.
Das T, Zaidi F, Farag M, Ruff KM, Messing J, Taylor JP, Pappu RV, Mittag T
bioRxiv [Preprint]. 2024 Mar 3:2024.02.28.582569. doi: 10.1101/2024.02.28.582569.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234607 | A1-LCD D262V | Bacterial expression of N-terminally 6His tagged hnRNPA1 D262V LCD (186-320) |
| 234608 | A1-LCD D262N | Bacterial expression of N-terminally 6His tagged hnRNPA1 D262N LCD (186-320) |
| 234609 | A1-LCD allW | Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact. |
| 234610 | A1-LCD -4D+4V | Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to valine |
| 234611 | A1-LCD -4D+4N | Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to asparagine |
| 234612 | A1-LCD -3G1S+4V | Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 Gly and 1 Ser mutated to Asn |
| 234613 | A1-LCD -3D+3V | Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to valine |
| 234614 | A1-LCD -3D+3N | Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to asparagine |
| 234615 | A1-LCD allW D262N | Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262N with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact. |
| 234616 | A1-LCD allW D262V | Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact. |
| 234617 | A1-LCD 5W D262V | Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with partial W mutant |
| 234618 | A1-LCD 11W D262V | Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with partial W mutant |
| 234619 | hnRNPA1-FLAG-IRES-eGFP | Mammalian expression of full-length hnRNPA1 with C-terminal FLAG tag co-expressing with eGFP via IRES sequence |
| 234620 | hnRNPA1_D262V-FLAG-IRES-eGFP | Mammalian expression of full-length hnRNPA1 D262V with C-terminal FLAG tag co-expressing with eGFP via IRES sequence |
| 234621 | hnRNPA1_allW_D262V-FLAG-IRES-eGFP | Mammalian expression of full-length hnRNPA1 D262V with all aromatic amino acids in the C-terminal LCD mutated to W keeping the hexapeptide fibril core (SYNDFG) and the PY-NLS intact. |