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Addgene

Population-level amplification of gene regulation by programmable gene transfer.

Son HI, Hamrick GS, Shende AR, Kim K, Yang K, Huang TJ, You L
Nat Chem Biol. 2025 Jan 8. doi: 10.1038/s41589-024-01817-9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
238033ADEPT-pTargetEncodes sfGFP-SsrA under constitutive promoter (J23119). Expresses a single guide RNA, which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
238034ADEPT-pTarget-Msp1Encodes sfGFPunder lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.
238035ADEPT-pTarget-Msp2Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.
238036ADEPT-pTarget-Msp3Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.
238037ADEPT-pTarget-Msp4Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.
238038ADEPT-pTarget-Msp5Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.
238039ADEPT-pTarget-Msp6Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.
238043ADEPT-pTarget-tetRModified ADEPT-pCas9 with sfGFP under TtrB promoter for tetrathionate (TTR) sensing.

Antibodies from Article