Population-level amplification of gene regulation by programmable gene transfer.
Son HI, Hamrick GS, Shende AR, Kim K, Yang K, Huang TJ, You L
Nat Chem Biol. 2025 Jan 8. doi: 10.1038/s41589-024-01817-9.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
238033 | ADEPT-pTarget | Encodes sfGFP-SsrA under constitutive promoter (J23119). Expresses a single guide RNA, which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance. |
238034 | ADEPT-pTarget-Msp1 | Encodes sfGFPunder lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance. |
238035 | ADEPT-pTarget-Msp2 | Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance. |
238036 | ADEPT-pTarget-Msp3 | Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance. |
238037 | ADEPT-pTarget-Msp4 | Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance. |
238038 | ADEPT-pTarget-Msp5 | Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance. |
238039 | ADEPT-pTarget-Msp6 | Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance. |
238043 | ADEPT-pTarget-tetR | Modified ADEPT-pCas9 with sfGFP under TtrB promoter for tetrathionate (TTR) sensing. |