Population-level amplification of gene regulation by programmable gene transfer.

Son HI, Hamrick GS, Shende AR, Kim K, Yang K, Huang TJ, You L
Nat Chem Biol. 2025 Jan 8. doi: 10.1038/s41589-024-01817-9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
238032	ADEPT-pCas9	Encodes Cas9 (ssrA degradation-tagged) under a tetracycline promoter (pTet) on a low-copy replication origin (SC101) as part of the ADEPT system.
238033	ADEPT-pTarget	Encodes sfGFP-SsrA under constitutive promoter (J23119). Expresses a single guide RNA, which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
238034	ADEPT-pTarget-Msp1	Encodes sfGFPunder lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.
238035	ADEPT-pTarget-Msp2	Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.
238036	ADEPT-pTarget-Msp3	Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.
238037	ADEPT-pTarget-Msp4	Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.
238038	ADEPT-pTarget-Msp5	Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.
238039	ADEPT-pTarget-Msp6	Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.
238040	ADEPT-pTarget-RFP	Encodes mRFP1 under pLux promoter. Expresses a single guide RNA (under Ara promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
238041	ADEPT-pTarget-GFP	Encodes sfGFP under lac promoter. Expresses a single guide RNA (under Rha promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
238043	ADEPT-pTarget-tetR	Modified ADEPT-pCas9 with sfGFP under TtrB promoter for tetrathionate (TTR) sensing.