Periodic ER-plasma membrane junctions support long-range Ca(2+) signal integration in dendrites.
Benedetti L, Fan R, Weigel AV, Moore AS, Houlihan PR, Kittisopikul M, Park G, Petruncio A, Hubbard PM, Pang S, Xu CS, Hess HF, Saalfeld S, Rangaraju V, Clapham DE, De Camilli P, Ryan TA, Lippincott-Schwartz J
Cell. 2025 Jan 23;188(2):484-500.e22. doi: 10.1016/j.cell.2024.11.029. Epub 2024 Dec 20.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 236228 | pAAV hSyn INTRON mEmerald-Sec61b WPRE | AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to mEmerald. |
| 236229 | pAAV hSyn INTRON HaloTag-Sec61b WPRE | AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to HaloTag |
| 236230 | pAAV hSyn INTRON mEmerald-ER WPRE | AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum |
| 236231 | pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE | AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2 |
| 236232 | pAAV hSyn INTRON mScarlet-CAAX2 WPRE | AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2 |
| 236233 | pAAV hSyn INTRON HaloTag-STIM1 WPRE | AAV expression of mouse STIM1 internally tagged with HaloTag |
| 236234 | pAAV hSyn INTRON mEmerald-STIM1 WPRE | AAV expression of mouse STIM1 internally tagged with mEmerald |
| 236235 | pAAV hSyn INTRON HaloTag-STIM2 WPRE | AAV expression of human STIM2 internally tagged with HaloTag |
| 236236 | pAAV hSyn INTRON mScarlet-STIM2 WPRE | AAV expression of human STIM2 internally tagged with mScarlet-I |
| 236237 | pAAV hSyn INTRON mCherry-Jph3 WPRE | AAV expression of human Junctophilin3 N-terminally tagged with mCherry |
| 236238 | pAAV hSyn INTRON HaloTag-Jph3 WPRE | AAV expression of human Junctophilin3 N-terminally tagged with HaloTag |
| 236239 | pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE | Lentiviral expression of voltage-gated calcium channel, CaV2.1, N-terminally tagged with mEmerald |
| 236240 | pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE | Lentiviral expression of voltage-gated calcium channel, CaV2.2, N-terminally tagged with mEmerald |
| 236241 | pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE | Lentiviral expression of voltage-gated calcium channel, CaV1.2, N-terminally tagged with mEmerald |
| 236242 | pLentiSIV hSyn HA-NLS-SpCas9-NLS WPRE | Lentiviral expression of SpCas9 |
| 236243 | SIV_Gag_Pol | 3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Gag and Pol |
| 236244 | SIV_Rev | 3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Rev |
| 236245 | pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2 | Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter |
| 236246 | pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2 | Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane |