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Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery.

Baysal C, Kausch AP, Cody JP, Altpeter F, Voytas DF
Plant J. 2025 Jan;121(2):e17196. doi: 10.1111/tpj.17196. Epub 2024 Dec 11. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
234811pEE082 FoMVTo clone guide RNA (gRNA) along with its scaffold in to the Foxtail Mosaic Virus Vector (T-DNA)
234812pJPC596 FoMV-AmCyanTo express flourescent protein AmCyan from the Foxtail Mosaic Virus Vector (T-DNA)
234813pCB036 FoMV-sgGFPTo express gRNA from Foxtail Mosaic Virus Vector targeting GFP (T-DNA)
234815pCB092 FoMV-sgMgChTo express guide gRNA from Foxtail Mosaic Virus Vector targeting SbMgCh (T-DNA)
234816pCB094 FoMV-sgLwTo express guide gRNA from Foxtail Mosaic Virus Vector targeting SbLw (T-DNA)
234818pGY037 BSMV-gamma-AmCyan To express flourescent protein AmCyan from the gamma genome of Barley Stripe Mosaic Virus Vector (T-DNA)

Antibodies from Article