Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery.
Baysal C, Kausch AP, Cody JP, Altpeter F, Voytas DF
Plant J. 2025 Jan;121(2):e17196. doi: 10.1111/tpj.17196. Epub 2024 Dec 11.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
234811 | pEE082 FoMV | To clone guide RNA (gRNA) along with its scaffold in to the Foxtail Mosaic Virus Vector (T-DNA) |
234812 | pJPC596 FoMV-AmCyan | To express flourescent protein AmCyan from the Foxtail Mosaic Virus Vector (T-DNA) |
234813 | pCB036 FoMV-sgGFP | To express gRNA from Foxtail Mosaic Virus Vector targeting GFP (T-DNA) |
234815 | pCB092 FoMV-sgMgCh | To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbMgCh (T-DNA) |
234816 | pCB094 FoMV-sgLw | To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbLw (T-DNA) |
234818 | pGY037 BSMV-gamma-AmCyan | To express flourescent protein AmCyan from the gamma genome of Barley Stripe Mosaic Virus Vector (T-DNA) |